Quantitative Polymerase-Kettenreaktion (qPCR)-basierte Schnelldiagnose von Helicobacter pylori-Infektion und Antibiotikaresistenz

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Zitiert von 8

06:40 Min.

28. Juli 2023

10.3791/65689-v

28. Juli 2023

5.7K Aufrufe

Liang Wang^*¹^,²^,³ , Jin-Xin Lai^*¹ , Yu-Ting Si^*¹^,⁴ , Xu-Xia Cui¹^,⁴ , Zeeshan Umar¹^,⁵ , Xiao-Jun Ru¹ , Xin-Yu Zhang¹ , Zheng-Kang Li¹ , Alfred Chin Yen Tay⁵^,⁶^,⁷^,⁸ , Barry J. Marshall⁵^,⁶^,⁷^,⁸ , Guang-Hua Li¹ , Bing Gu¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ²Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, ³Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, ⁴Medical Technology School of Xuzhou Medical University, ⁵Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, ⁶The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, ⁷Marshall International Digestive Diseases Hospital, Zhengzhou University, ⁸Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

^* These authors contributed equally

Das Protokoll stellt eine nicht-invasive Methode zur schnellen Diagnose von Helicobacter pylori-Mageninfektionen durch den String-Test vor und bestimmt die Antibiotikaresistenz gegen Clarithromycin und Levofloxacin mittels quantitativer Polymerase-Kettenreaktion (qPCR).