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Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

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Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

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Biologie

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JoVE Journal Biologie

Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells

Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

Quantitative Analysis of Mitophagy in Murine Pancreatic β-Cells

DOI:

10.3791/65789-v

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01:09 min

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September 15, 2023

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Elena Levi-D’Ancona², Vaibhav Sidarala, Scott A. Soleimanpour^3,4

¹Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,University of Michigan, Ann Arbor, ²Graduate Program in Immunology,University of Michigan Medical School, ³Department of Molecular and Integrative Physiology,University of Michigan, Ann Arbor, ⁴VA Ann Arbor Healthcare System

Tags

Mitophagy Beta-cells Flow Cytometry Mt-Keima MtPhagy Mitochondrial Function Diabetes Quantification Genetic Reporter Dye-based Method

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Author Spotlight: Assessment of Mitophagy Flux in Pancreatic β-Cells Using Effective and Robust Complementary Approaches

Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using MtPhagy Dye

Flow Cytometric Analysis of Mitophagy in Murine Pancreatic β-Cells Using the Genetically Encoded mt-Keima Reporter

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