This manuscript describes a detailed protocol for isolating retinal glial Müller cells from mouse eyes. The protocol starts with enucleation and dissection of mouse eyes, followed by isolation, seeding, and culturing of Müller cells.
Tomaszewski, R., Gad, M. S., Negoita, P., Abdelkarim, R., Tawfik, A. Isolation of Primary Mouse Retinal Glial Müller Cells. J. Vis. Exp. (210), e66237, doi:10.3791/66237 (2024).