Rodent Models

This video demonstrates a procedure to inject microbubble contrast agents into an isolated late gestation stage mouse embryo. The microbubble contrast agent-based ultrasound imaging helps visualize and characterize the vascular environment of the embryo.

This video demonstrates the retrieval of embryos from a pregnant mouse. The recovered perinatal embryos can be used as model systems for studying mammalian development.

In this video, we administer methylene blue dye injection into the urinary tract of a perinatal embryonic mouse to assess congenital anomalies related to urinary tract obstruction.

This video demonstrates the transuterine tracheal occlusion procedure of a mouse fetus in a pregnant mouse. This model can be used to study the impact of occluded trachea on lung development.

In this video, we describe a surgical procedure to isolate ovaries from a neonatal mouse.

In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.

In this video, we demonstrate the procedure to surgically remove the left ventricular apex of a neonatal mouse heart.

This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.

This article describes a way to prepare and insert dihydrotestosterone (DHT) pellets to model Polycystic Ovary Syndrome (PCOS) in mice. The example protocol demonstrates the procedure in mice used for reproductive and metabolic studies.

This video shows how to use the Barnes Maze with a mouse. The Barnes Maze testing procedure is used by researchers to assess spatial learning and memory in laboratory animals following repeated trials.

In this video, we describe the footprint testing procedure and related analysis to assess gait and motor coordination in mice.

This video presents the procedure for inducing status epilepticus (SE) in mice by systemic administration of pilocarpine to model temporal lobe epilepsy.

In this video, we perform the accelerating rotating rod or rotarod test to assess motor coordination and learning in mouse...

In this video, we describe the Morris water maze test with platform relocation, a navigational task used to study working memory in rodents.

This video describes the open field behavior test used to examine general locomotion and exploration habits in...

This video describes a procedure for mouse liver perfusion through the portal vein using collagenase solution prior to isolating liver cells.

This video shows a headpost implantation surgery in murine model. The headpost is used not only to head-fix and therefore restrain the mouse during experimentation, but also to securely attach a head device in freely moving animals.

This video describes a protocol to perform echocardiography on a laboratory mouse.

This video demonstrates a method of producing decellularized extracellular matrix or ECM scaffolds by applying antegrade perfusion of detergents through the vasculature. The structural ECM scaffolds can then be used to analyze ECM remodeling associated with cardiopulmonary disease.

This video demonstrates a surgical procedure to dissect the palate tissue from a murine model.

In this video, we describe the isolation of circumvallate papillae (CVP) epithelium from a murine tongue.

In this video, we describe a surgical procedure to generate a mouse model of myocardial infarction by permanently ligating the left anterior descending coronary artery.

In this video, we demonstrate high-frequency ultrasonography to image embryo implantation sites in a pregnant murine model. This imaging technique successfully detects viable pregnancy and characterizes embryo-implantation sites during early murine pregnancy.

This video describes a swab-based technique to isolate viable commensal bacteria from the ocular conjunctiva. The technique can be used to profile the commensal bacterial microbiome of the conjunctiva, which plays a defensive role against pathogens.

In this video, we describe a surgical procedure to implant a temperature telemetric device  in a mouse model to continuously measure its core body temperature.

In this video we describe a step-by-step protocol to study exertional heat stroke (EHS) in a mouse model.

This video describes an imaging procedure to observe and study intravascular dynamics of a mouse liver tissue using multiphoton microscopy following stabilization of the organ. Multiphoton microscopy enables the imaging of biological tissue at high spatial resolution in vivo.

In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.

This video describes the procedure for the generation of a mouse model with acute biliary pancreatitis via pancreatic infusion of sodium taurocholate. This model helps study pathophysiological changes induced by sodium taurocholate in the pancreas.

In this video, we demonstrate the isolation of aortic valve interstitial cells from the heart of an adult mouse using a two-step collagenase procedure and their subsequent culture in low volume of growth media.

In this video, we demonstrate an experimental protocol for minimally invasive transverse aortic constriction in mice to examine pressure overload-induced heart failure.

This video demonstrates the technique of aortic debanding, which can lead to reverse remodeling to limit the progression towards heart failure in a murine model. Post debanding, a significant reduction of ventricular afterload triggers regression of ventricular hypertrophy and fibrosis, causing a recovery in cardiac function.

This video describes the isolation of antral oocytes from freshly harvested mouse ovaries. These antral oocytes can be used for further characterization and developmental studies.

This video describes the procedure for biliary system resin casting, for understanding 3D biliary architecture. This technique provides insights into the internal diameter and network connectivity of the biliary system, which could be used for liver regeneration analysis.

This video demonstrates the procedure to generate photothrombotic stroke in a mouse model by laser illumination of an intact skull following administration of photosensitive dye.

In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.

This video demonstrates the procedure to isolate sinoatrial node or SAN from mouse heart. The SAN houses the sinoatrial cells that initiates heartbeat by generating spontaneous action potentials.

In this video, we demonstrate the assembly of a micro-osmotic pump brain infusion system followed by its surgical implantation in the mouse skull. This system enables continuous delivery of potential drugs and facilitates the study of their effects on the brain and brain tissue.

In this video, we describe a surgical procedure to harvest intact ovary from a mouse model.

In this video, we describe a method for implanting a catheter into the jugular vein of a mouse model through which experimental agents such as drugs and tracer dyes can be delivered intravenously in vivo. Such an arrangement when connected to other systems can also be used for monitoring hemodynamics and blood sampling.

In this video, we describe a surgical procedure to implant a cranial ring assembly on the exposed skull bone and further immobilize the mouse’s head for high-resolution brain imaging for a long duration.

This video demonstrates the procedure to implant a pancreatic imaging window to separate the pancreas from the intestines for longitudinal time-lapse high-resolution imaging of the pancreas microstructure.

In this video, we describe the intravital imaging of a mouse pancreas through a stabilized window for long-term visualization of cellular dynamics.

This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.

In this video, we demonstrate the technique to image the inguinal lymph node in a mouse model using ultra-high-frequency ultrasound imaging. This non-invasive imaging technique allows the monitoring of the change in lymph node volume in the animal over time.

This video describes a protocol to isolate the retinal cup without the outer covering layers from the eyeball of a mouse. The extracted retinal tissue can help study various metabolic pathways occurring in the retinal cells.

In this video, we describe the procedure to isolate and chemically fix the mouse pulmonary value in closed conformation via perfusion pressurization.

This video describes an in vivo technique to generate murine tail lymphedema, an abnormal fluid accumulation in the interstitial tissue space leading to swelling in the tail. The model is applied to study mechanisms and therapy for lymphedema.

In this video, we describe the technique for the intravesical administration of uropathogenic bacteria to generate a urinary tract infection model in mice. This model can be used to understand the spread of uropathogenic bacterial infection within the urinary system and the mechanisms that determine UTI susceptibility.

This video describes the surgical procedure to immobilize the laser Doppler probe over the murine brain's middle cerebral artery, or MCA. The probe helps to measure the blood flow in the brain's microvasculature by measuring the Doppler shift caused due to the motion of red blood cells within the artery.

This video describes the procedure to develop transient stroke in a murine model using silicon-coated filament-based occlusion of the middle cerebral artery. The developed model mimics human stroke, thus helping to investigate novel therapeutic strategies for treating stroke.

In this video, we describe the surgical procedure to induce lumbar spine instability in a mouse model by resecting the Lumbar L3 to L5 spinous process along with supraspinous and interspinous ligaments.

This video demonstrates the protocol for performing colonoscope-guided pinch biopsy in the mouse. This technique helps visually analyze the colon lumen and collect tissue samples for detailed studies.

In this video, we describe a procedure to isolate the colonoscopic-induced wound bed at an appropriate time point for detailed molecular analysis. This analysis helps to understand the wound healing process in inflammatory conditions of the GI tract.

This video demonstrates the generation of an ileal loop model to study intestinal permeability in a mouse model. This method provides a live model to understand the epithelial barrier function and consequent immune response.

In this video, we describe a surgical procedure to generate mouse proximal colon loop or pcLoop segment followed by intraluminal injection of fluorescent markers into the segment to study the intestinal epithelial permeability.

This video demonstrates the isolation of single myofibres from harvested murine Extensor Digitorum Longus (EDL) muscle. The isolated myofibres can be cultured to study myofiber-associated muscle stem cell activity.

This video describes a bariatric surgery-Roux-en-Y procedure. This involves the cutting of the jejunum- the middle portion of the intestine- to generate two intestinal limbs. The biliary limb is attached to the existing intestinal loop and the alimentary limb is attached to the stomach, creating a gastric bypass. The gastric bypass created here helps to decrease the nutrient uptake and efficiently reduces body weight.

This video describes a method for generating a mouse model that mimics bacterial endophthalmitis – an intraocular infection resulting from a bacterial invasion into the eye’s vitreous cavity – via intravitreal injection of a bacterial suspension into the eye. This mouse model helps study the disease mechanism and evaluate therapeutics for it.

This video describes the technique of quantifying bacterial infection from eye homogenates obtained from a murine model of bacterial endophthalmitis caused by Bacillus cereus. This technique helps study the host immune response and its dependency on the host and ocular bacterial gene expression in murine models.