Roux-en-Y Gastric Bypass Procedure: A Bariatric Surgical Procedure for Gastric Resectioning in Murine Model

Begin the Roux-en-Y procedure — a gastric resection surgery — by placing an anesthetized mouse in the supine position. Dissect the abdomen and externalize the small intestine to locate the jejunum — the middle part of the intestine.

Perform two nearby ligations on the jejunum and make an excision between them. This process generates two intestinal limbs — the biliary limb is proximal to the stomach and the alimentary limb is distal to the stomach. Place the biliary limb near the existing intestinal loop. Make equal-sized longitudinal incisions on their antimesenteric surface.

Perform side-to-side anastomosis by suturing incised regions of the biliary limb and the intestinal loop, allowing the gastric juices to enter intestines directly. Locate the stomach and ligate the pylorus — the portion of the stomach that opens into the intestine, which prevents the food from entering the intestine.

Make a new longitudinal incision near the pylorus and the pulled end of the alimentary limb and perform the second side-to-side anastomosis. This new path is called the Roux limb, and the intestine now resembles ‘Y’ shape. Return the ligated organs to the abdominal cavity and suture the incision s