Danio rerio (zebrafish)

In this article, zebrafish embryos are used for chemical toxicity screening. Chemical toxicity is correlated with phenotype readouts. The sample protocol shows a methodological approach of performing the chemical toxicity screening.

The article describes a protocol to generate zebrafish embryos through natural mating and sorting their developmental stages. The protocol is used further for analysis of pancreatic β-cells by using transgenic fishes.

This article describes sample preparation for light-sheet fluorescence microscopy. It also demonstrates a method to mount live zebrafish embryos for long term imaging, which allows recording of long time-lapse movies of live embryos in near-physiological conditions.

This article describes a method to mount live zebrafish embryos for long term imaging. This method is cost effective and easy to perform using regular glass-bottom microscopy dishes for imaging on Inverted Microscope. The mounting is performed in layers of agarose at different concentrations.

This video describes step by step instructions on mounting live zebrafish embryos on 6 well plate using low melt agarose. It also provides the process to acquire images of live embryos and analyze it using software.

This video describes the method to injure axons in zebrafish embryos using two photon laser axotomy and observing axonal recovery from injury.

This protocol describes a technique called whole-cell patch clamp electrophysiology, which is a method to study electrical properties of Mauthner neurons and other reticulospinal cells in zebrafish embryos.

This video describes a technique of Embryo microinjection to deliver compounds into Zebrafish yolk sac.

This video describes the photomotor response assay of zebrafish and fathead minnow larvae using an automated tracking software. The protocol measures the behavioral response of the larval fish following a sudden change in lighting condition.

This video describes the prey capture assay. The video discusses the method to study prey capture behavior of zebrafish larvae after the laser ablation of specific neurons.

This video describes the touch-evoke response assay, which measures the response of the zebrafish larva to touch stimuli.

The video describes the avoidance and thigmotaxis assay of zebrafish larvae. The video examines the behavior of zebrafish larvae in response to visual aversive stimulus.

This article describes zebrafish’s behavioral response to toxic compounds by monitoring larval locomotion. The protocol further looks at an example of zebrafish larvae’s response to environmental pollutant.

The article describes targeted liver ablation, a method for inducing hepatocyte death with metronidazole in transgenic zebrafish larvae. The sample protocol describes the process for generating, manipulating and assessing targeted liver ablation in zebrafish.

This video describes optogenetic techniques by activating genetically modified somatosensory neurons and recording elicited behavioral response with a high-speed video camera.

This video describes the protocol which is developed to dissect adult and larval zebrafish lenses to analyze cortical lens morphology and structural integrity of the lens sutures.

This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.

This video shows how to perform electrocardiography (ECG or EKG) measurements, a test which measures the electrical activity of the heart, on a zebrafish.

This video describes the rheotaxis assay in zebrafish. The method involves the measurement of orientation behavior of zebrafish in response to different water flow rates under the influence of different magnetic fields.

The video describes the light-dark preference assay to study the anxiety-related behavior in zebrafish following the exposure to ethanol.

This video describes the optokinetic response assay in zebrafish. The video measures the reflexive eye movements based on the visual moving stimulus and determines the visual acuity in zebrafish.

The article describes an assay for studying zebrafish’s aggression. The method uses a simple mirror and observes zebrafish’s behavioral response towards its own reflection in mirror.

The article describes a behavioral method to assess stress in zebrafish. In response to stress, zebrafish tends to swim at bottom of a tank until stress is removed. The sample protocol describes stepwise method for performing the assay.

The protocol describes targeted knockdown of gene expression in zebrafish by morpholino injection in the tail.

This article describes DNA extraction from zebrafish tail clip. The protocol demonstrates a rapid and efficient method to analyze zebrafish genotypes.

The protocol describes the procedure to perform electrophysiology in adult zebrafish. This method can be used for measuring neural activity in vivo.

This video describes in vivo electroporation, which is a technique for DNA delivery into ependymoglial cells in adult zebrafish.

This video describes the technique of intramuscular injection for delivering compounds into the dorsal muscles of an adult zebrafish.

This video describes a technique of intraperitoneal injection, a method for delivering solutions into the abdominal cavity of Adult Zebrafish.

This video describes the technique of delivering compounds into the heart of adult zebrafish by using intrathoracic injection.

This video describes the technique of isolating the intestine to evaluate the intestinal uptake of bioactive compounds. In this sample, we will isolate the intestine from an adult zebrafish to analyze the uptake of immunostimulants.

This video describes a technique of isolating the heart from a zebrafish at multiple developmental stages to study cardiac development, maturation, and ageing.

This video describes the technique of microdissection of the brain from an adult zebrafish. This method helps in obtaining whole brain derived neuorospheres that can be studied further.

This video describes the technique of creating a zebrafish cardiomyopathy model with minimal variations among experimental groups and in the sample, we create zebrafish cardiomyopathy model using the drug doxorubicin.

This video describes the technique of creating a zebrafish model for Type 1 diabetes mellitus with the help of the drug Streptozocin, to study the pathophysiology of diabetic complications and metabolic memory.