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- To begin, grow the embryos in 3 Petri dishes. The first one is a control plate that contains Hank's solution-- a balanced salt solution. The second and third plates have Hank's solution with a potentially neurotoxic compound at different concentrations.
The neurotoxic compound in the exposure solution penetrates the embryos' external membrane by passive diffusion and alters the nervous system's processes such as attention, coordination, and muscle activity. Once the embryos develop into larvae of the appropriate age, transfer each larva into an individual well of a 48 well plate. Keep track of the treatment condition for each animal.
Transfer the well plate on to a recording platform and pull down the cover. Let the larvae acclimate for 10 minutes. Set alternating cycles of light and dark periods using the software.
Zebrafish larvae normally exhibit more movement in the dark and less movement in the light. Record the total distance traveled by the larvae of the control and the exposure groups in each period. If the compound is neurotoxic, larvae show even more movement in the dark and in the light than control animals.
In the following protocol, we will perform behavioral tests o
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