Breast Cancer

In this article we discuss how to establish a breast cancer model in female mice by injecting breast cancer cells into the mammary fat pad. The example protocol demonstrates the orthotopic injection.

This video utilizes ultrasonography for diagnosis and follow-up tests to determine treatment efficacy of Progressive Resistance Exercise (PRE) in breast cancer-related lymphedema (BCRL). Ultrasonography can be applied effectively to ensure diagnosis and treatment of lymphedema.

The video demonstrates the breast cancer orthotopic inoculation technique in a mouse model. This technique produces a high tumorigenesis rate with less variability in tumor size and shape compared to subcutaneous or non-surgical injection.

This video describes a technique of radical mastectomy, which is used for the surgical removal of the entire mammary gland from a mouse to study cancer progression.

This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.

This video describes a microinjection method for breast cancer cell delivery into the perivitelline space of transgenic zebrafish embryos. After incubation, we visualize the embryo under a fluorescent microscope to observe cancer cell invasion, dissemination, and metastasis.

This video describes a surgical procedure to deliver mammary tumor cells to the murine liver via portal vein injection. This model permits the investigation of late stages of liver metastasis.

This video describes india ink inflation--a staining method--to quantify tumor nodules in tumor-bearing mouse lungs. After staining, the lungs are fixed in Fekete's solution to observe tumor nodules in the lungs.

This video describes a technique of measuring the interstitial fluid pressure within a tumor by perfusion imaging using an image-guided robotic system. Measurements are compared to the intra-tumoral accumulation of liposomes, within a solid tumor.

In this video we describe a technique of administering a solution of interest into the mammary duct of a rabbit and confirm the delivery by high-resolution ultrasound imaging of contrast agents.

This video describes the non-enzymatic dissociation of fresh human tissue to obtain viable cells. The technique is used for qualitative and quantitative analysis of CD45 positive cells (lymphocytes/leukocytes) present in various normal and malignant human tissues.

This video describes a technique of preparing breast cancer cell samples for metabolite profiling. Breast cancer cells are treated with estradiol, which binds to the estrogen receptor alpha and causes change in the metabolite...

This video describes a technique of injecting labeled cancer cells using tail vein injection into a mouse model. We further monitor breast cancer metastasis formation, and growth using real-time in vivo live animal imaging.

This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.

Source: Marina Arantes Radicchi1, Victor Carlos Mello1, Victória Paz Machado1, Márcia Cristina Oliveira da Rocha1, Jaqueline Vaz de Oliveira1, Luana Cristina Camargo1,2, Luís Alexandre Muehlmann1, Ricardo Bentes Azevedo1, Sônia Nair Bao1, João Paulo Figueiró Longo11Institute of Biological Sciences, University of Brasília, 2Department of Basic Psychological Processes, Institute of Psychology, University of BrasíliaThis video demonstrates the procedure for tracking tumor progression in vivo by...

This video describes isolating cancer stem cells from breast cancer cell lines. We culture these cancer stem cells to generate primary mammospheres for assessing self-renewal and calculating sphere-forming efficiency, allowing comparison across different seeding...

The following video describes a technique of transduction to label (patient-derived xenografts) PDX tumor cells, which is used for introducing lentivirus expressing green-fluorescent protein and luciferase reporters into the tumor cell in vitro.

This video describes the culturing of breast cancer cells adjacent to femur bone explants to measure cancer cell proliferation and colonization in a human bone tissue explant model system.

This video describes the 3D co-culture of breast cancer cells with monocytes to study their interaction in an extracellular matrix extract (ECME) based environment. This method helps in studying specific features such as collagen degradation, immune cell recruitment, and cell invasion.

This video describes a method of generating an organ conditioned medium derived from the mouse liver. This model system can be used to identify and study organ-derived soluble factors and their effects on the organ tropism and metastatic behavior of cancer cells.

This video provides a detailed methodology to grow breast cancer cells in a 3D basement membrane protein matrix, exemplifying the potential of 3D culture in the assessment of cell invasion into the surrounding environment.

This video describes a soft agar colony formation assay to test the effects of a PADI enzyme inhibitor and BB-Cl-amidine on breast cancer tumorigenicity in vitro. This assay also enables the quantitative assessment of cell transformation potential within the context of genetic perturbations.