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Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

February 2nd, 2024
1, 2, 2, 1,3, 1,4,5
1Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Cell Biology and Functional Genomics, 2Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, Columbia University, 3Department of Diabetes, Imperial College Healthcare NHS Trust, 4Faculté de Médicine, Université de Montréal, 5Lee Kong Chian Imperial Medical School, Nanyang Technological University

Summary
Automatic Translation

Denna artikel presenterar ett protokoll för riktad differentiering och funktionell analys av β-cellsliknande celler. Vi beskriver optimala odlingsbetingelser och passager för humana pluripotenta stamceller innan de genererar insulinproducerande bukspottkörtelceller. Differentieringen i sex steg fortskrider från definitiv endodermbildning till funktionella β-cellsliknande celler som utsöndrar insulin som svar på glukos.

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