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Novel ROS Quantification Using Dihydroethidium in Liver Cancer Cells

January 19th, 2024
1,2, 1,2
1Department of Pathology, University of New Mexico, 2Department of Chemical and Biological Engineering, University of New Mexico

Summary
Automatic Translation

This protocol describes a novel method to quantify intracellular reactive oxygen species (ROS) using dihydroethidium (DHE) as a fluorescence dye probe using a high-throughput screening approach. The protocol describes the methods for quantitative assessment of intracellular reactive oxygen species (ROS) in the three different hepatocellular carcinoma cell lines.

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