Shinshu University View Institution's Website 2 articles published in JoVE Cancer Research Lung Tumor Cell Recruitment Assay Takeshi Tomita1,2, Katsuaki Ieguchi1, Atsuko Deguchi1, Morichika Takita1, Fujiko Tsukahara1, Sachie Hiratsuka1,2, Yoshiro Maru1 1 This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis. Immunology and Infection piggyBac Transposon System Modification of Primary Human T Cells Sunandan Saha1,2, Yozo Nakazawa3, Leslie E. Huye4,5, Joseph E. Doherty2,6, Daniel L. Galvan2, Cliona M. Rooney4,5,7, Matthew H. Wilson1,2,4,8 1Program in Translational Biology and Molecular Medicine, Baylor College of Medicine, 2Department of Medicine, Division of Nephrology, Baylor College of Medicine, 3Department of Immunology and Pathology, Shinshu University School of Medicine, 4Center for Cell and Gene Therapy, Baylor College of Medicine, 5Department of Pediatrics, Baylor College of Medicine, 6Program in Cell and Molecular Biology, Baylor College of Medicine, 7Department of Molecular Virology and Microbiology, Baylor College of Medicine, 8Michael E. DeBakey VA Medical Center We describe a method to genetically modify primary human T cells with a transgene using the non-viral piggyBac transposon system. T cells modified to using the piggyBac transposon system exhibit stable transgene expression.