Nara Institute of Science and Technology 1 article published in JoVE Neuroscience Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance Takunori Minegishi1, Ryosuke Fujikawa2, Ria Fajarwati Kastian1, Yuichi Sakumura2, Naoyuki Inagaki1 1Laboratory of Systems Neurobiology and Medicine, Division of Biological Science, Nara Institute of Science and Technology, 2Laboratory of Data-Driven Biology, Division of Biological Science, Nara Institute of Science and Technology To advance, growth cones must exert traction forces against the external environment. The generation of traction forces is dependent on actin dynamics and clutch coupling. The present study describes methods for analyzing actin dynamics, clutch coupling and traction forces for growth cone advance.