Photon etc 1 article published in JoVE Chemistry Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals Emille M. Rodrigues1, Nelson Rutajoga1, David Rioux2, Jacob Yvon-Leroux2, Eva Hemmer1 1Department of Chemistry and Biomolecular Sciences, University of Ottawa, 2Photon etc Here, we present a protocol to obtain luminescent hyperspectral imaging data and to analyze optical anisotropy features of lanthanide-based single crystals using a Hyperspectral Imaging System.