Qiagen Sciences, Inc. 1 article published in JoVE Biology Pyrosequencing for Microbial Identification and Characterization Patrick J. Cummings1, Ray Ahmed2, Jeffrey A. Durocher2, Adam Jessen2, Tamar Vardi2, Kristina M. Obom1 1Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, 2Qiagen Sciences, Inc. Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.