Name: ligand binding and allosteric effects
Uploaded: 2023-04-30T18:28:41.000Z
Duration: 49 s
Channel: JoVE

K12 Standards

north-carolina-k-12-science-standard-course-of-study

North Carolina K-12 Science Standard Course of Study

9-12

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms- Structures and Processes

LS.Bio.1.2

Ligand Binding and Linkage

La mayoría de las proteínas tienen múltiples ubicaciones donde los ligandos pueden asociarse, además del sitio responsable de la función de la proteína. La unión de una molécula a cualquiera de estos sitios a menudo resulta en cambios conformacionales, alterando la forma de la proteína.
Cuando este cambio afecta a la unión de otro ligando, los dos sitios se describen como acoplados o enlazados. La unión del sitio de unión puede estar regulada positiva o negativamente.
En un enlace positivo, la unión de un ligando da lugar a cambios conformacionales que hacen que otro sitio sea más probable que se una a su ligando respectivo. 
En un ligamiento negativo, la unión de un ligando da lugar a cambios conformacionales que impiden que otro sitio de unión se asocie con su ligando.

<ol>
	<li>Suplatov, D., & Švedas, V. (2015). Estudio de sitios funcionales y alostéricos en superfamilias de proteínas. Acta naturae, 7(4), 34-45.</li>
	<li>Bellelli, A. y Carey, J. (2017). Proteínas con múltiples sitios de unión. En Unión de ligandos reversibles (eds. A. Bellelli y J. Carey). doi:10.1002/9781119238508.ch4</li>
	<li>Helmstaedt, K., Krappmann, S., & Braus, G. H. (2001). Regulación alostérica de la actividad catalítica: Escherichia coli aspartato transcarbamoilasa frente a la levadura corismato mutasa. Revisiones de microbiología y biología molecular : MMBR, 65(3), 404–421. doi:10.1128/MMBR.65.3.404-421.2001</li>
</ol>

unión y enlace de ligandos

Las proteínas alostéricas tienen más de un sitio de unión a ligando. La unión de un ligando a cualquiera de estos sitios influye en la unión de los ligandos a los otros sitios. Cuando una proteína es alostérica, sus sitios de unión se denominan acoplados o unidos. En el caso de las enzimas, el sitio que se une al sustrato se conoce como sitio activo y el otro sitio se conoce como sitio regulador. Cuando un ligando se une al sitio regulador, esto conduce a cambios conformacionales en la proteína que pueden influir en el sitio activo de la proteína.

Los enlaces de los sitios de unión pueden causar una regulación positiva o negativa de la unión del ligando a otros sitios. En los casos en los que ambos ligandos prefieren unirse a la misma conformación de una proteína, la unión en un sitio aumenta la afinidad del otro sitio por su respectivo ligando. Esto se conoce como vínculo positivo. Por otro lado, si los ligandos prefieren unirse a diferentes conformaciones, la unión de un ligando dificultará que el segundo ligando se una a la proteína. Esto se conoce como vínculo negativo.

Unión y enlace de ligandos

Las proteínas alostéricas tienen más de un sitio de unión al ligando; La unión de un ligando a cualquiera de estos sitios influye en la unión de los ligandos a los otros ...

Education

Educación

JoVE Core

Cell Biology

Biología Celular

Unit 1

Unidad 1

Energy and Catalysis

Energía y Catálisis

KEY TERMS AND CONCEPTS

TÉRMINOS Y CONCEPTOS CLAVE

ligand binding and allosteric effects

Ligand binding and linkage describe how one molecule can affect another when it binds to a protein. In this topic, the focus is on how binding at one site can influence activity at a different site on the same protein.

This idea is important for understanding protein function in high school biology. It helps explain why some proteins respond more strongly after a ligand attaches. The transcript centers on the connection between ligand binding and linkage, which is a key concept in molecular interactions.

The term ligand means a molecule that binds to a protein. Linkage describes the way that this binding is connected to changes elsewhere in the protein. Together, these ideas show how protein behavior can be controlled by molecular binding events.

Ligand Binding and Allosteric Effects

the first law of thermodynamics

Energy Changes in Cells and Matter

The first law of thermodynamics describes how energy changes form in the universe. The total amount of energy stays constant. Energy can move from one place to another or change into a different form, but it cannot be created or destroyed. These energy changes happen in everyday life. A light bulb changes electrical energy into light energy. A gas stove changes chemical energy in natural gas into heat energy. Plants also carry out a major energy transformation when they convert sunlight energy...

the second law of thermodynamics

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the equilibrium binding constant and binding strength

the-equilibrium-binding-constant-and-binding-strength

How Equilibrium Binding Constant Affects Binding

The equilibrium binding constant describes how strongly two molecules bind at equilibrium. It is a useful way to compare binding strength in molecular interactions. This topic focuses on how binding is measured and what the equilibrium binding constant means. A higher value indicates stronger binding, while a lower value indicates weaker binding. Understanding this constant helps explain how tightly molecules stay together under equilibrium conditions. The page also connects the binding...

free energy and equilibrium

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How Enzymes Change Shape to Catalyze

Enzyme catalysis depends on how enzymes bind substrates and how the active site responds during the reaction. Scientists once described this process with a simple lock-and-key model, where the enzyme and substrate fit together in one instant. Research now supports induced fit, a more dynamic model in which binding causes a small change in the enzyme’s shape. That shift helps create an ideal match between the enzyme and the substrate’s transition state, which improves catalysis. The active site...

ligand binding and linkage

Ligand binding and linkage describe how one molecule can affect another when it binds to a protein. In this topic, the focus is on how binding at one site can influence activity at a different site on the same protein. This idea is important for understanding protein function in high school biology. It helps explain why some proteins respond more strongly after a ligand attaches. The transcript centers on the connection between ligand binding and linkage, which is a key concept in molecular...

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key terms and concepts

Learn how ligand binding can change protein activity through linkage, where binding at one site affects another site on the same protein.

Ligand Binding and Allosteric Effects | JoVE K12

cell biology

JoVE Core Biología Celular ilustra procesos celulares complejos mediante completos vídeos animados fáciles de entender. Esta colección se centra en los componentes esenciales de las células y su funcionamiento, incluyendo los orgánulos y las membranas, el transporte celular y el metabolismo energético celular. Todo este material se complementa con los vídeos de científicos en acción, que muestran la aplicación de la biología celular en experimentos reales de investigación.

Virginia  Standards of Learning

HIgh School

BIO.2

BIO.2.c

Florida Department of Education

High School

Life Science

SC.912.L.18

SC.912.L.18.4

SC.912.L.18.11

Texas Essential Knowledge and Skills

BIO.11

BIO.11.B

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Ligand Binding and Allosteric Effects

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