Assessing the Antibacterial Activity of Nanostructured Surfaces Using a Focused-Contact Exposure Method

Resumen

Source: Holt-Torres, P. S. et al. Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro. J. Vis. Exp. (2023)

This video demonstrates the use of the focused-contact exposure method to evaluate the antibacterial activity of nanostructured surfaces. It outlines the steps involved in establishing direct contact between bacteria and the nanostructured surface, incubation, bacterial collection, and colony counting to assess viability.

Protocolo

1. Focused-contact exposure method (method D)
NOTE: In method D, bacteria on a nitrocellulose filter paper are put in direct contact with an area of interest on the nanostructured surfaces. This method minimizes the interference of bulk sample degradation in bacterial cultures with the bacterial activities.

1. Create a bacterial seeding culture and confirm the actual seeding density.
2. Prepare the samples to a size of 1 × 1 cm² in a square shape. Prepare all the sample types in triplicate. If necessary, place the samples on a three-dimensional (3D) holder that is 15 mm in diameter by 10 mm in height. Place the sample and....

Materiales

Lista de materiales utilizados en este artículo

Nombre	Empresa	Número de catálogo	Comentarios
Tris(hydroxymethyl) aminomethane buffer pH 8.5; Tris buffer	Sigma-Aldrich	42457
Falcon 15 mL conical tubes	Fisher Scientific	14-959-49B
Luria Bertani Broth	Sigma Life Science	L3022
Luria Bertani Broth + agar	Sigma Life Science	L2897
MacroTube 5.0	Benchmark Scientific	C1005-T5-ST
Nitrocellulose paper	Fisherbrand	09-801A
Petri dish 100 mm	VWR	470210-568
Petri dish, 15 mm	Fisherbrand	FB0875713A
Sonicator	VWR	97043-936
VWR Benchmark Incu-shaker 10L	VWR	N/A