All procedures involving human participants were conducted in accordance with the Declaration of Helsinki and institutional requirements. The Ethics Committee of Wuhan Sports University approved the protocol (approval number 2023070). All participants provided written informed consent. The approved consent framework permitted public sharing of the deposited deidentified research data.
Participants and cohort characterization
Eighteen female collegiate basketball players from the same team roster who were available to complete all three experimental conditions were recruited. All athletes were classified as National First-Class athletes and completed the protocol during the January 2026 preseason preparatory phase. The sample represented the available eligible roster; no prospective sample size or power calculation was conducted. Age, height, body mass, body-fat percentage, training experience, playing position, competitive classification, habitual daytime-nap behavior, habitual caffeine or energy-drink use, and hormonal-contraceptive use were recorded. All participants in this cohort self-reported a regular daytime-nap habit; none reported habitual coffee or energy-drink consumption, and none used hormonal contraceptives.
The complete Chinese-language 19-item Morningness–Eveningness Questionnaire, Pittsburgh Sleep Quality Index, and 18-item Athlete Sleep Behavior Questionnaire-2 were administered during each athlete’s N0 session. Participants completed the questionnaires independently, and the study team scored them using the Chinese-language scoring instructions used for the project13,14,15. These scores were treated as descriptive measures of recent or habitual sleep-related characteristics, not as day-level exposure measures or randomized covariates. Previous-night sleep duration, experimental-nap duration, menstrual-cycle phase, and menstrual symptoms were not inferred from these questionnaires. In the present study, previous-night sleep logs, day-level sleepiness or vigilance, menstrual-cycle phase, and menstrual symptoms were not recorded.
Randomization, sequence balance, and condition spacing
A randomized, fully counterbalanced, three-period within-participant crossover design was used. The conditions were defined as N0 (no nap seated wakefulness), N30 (30 min scheduled afternoon nap opportunity), and N90 (90 min scheduled afternoon nap opportunity). The six possible sequences—N0–N30–N90, N0–N90–N30, N30–N0–N90, N30–N90–N0, N90–N0–N30, and N90–N30–N0—were assigned to three athletes each. Allocation was determined by lot drawing. Contemporaneous staffing documentation was not preserved, so personnel-role details and assessor-masking status could not be independently verified from a source record.
Successive condition days for each athlete were separated by at least 72 h. All three conditions were completed during the same preseason training phase, in the same indoor basketball arena, on the same wooden court, within the same afternoon time window, and with the same test order, equipment positions, air-conditioning settings, footwear, and testing personnel. The same pair of basketball shoes was used by each athlete across all three conditioning days. Venue and environmental control procedures were standardized, but day-level temperature and humidity data were not archived.
Participants necessarily knew whether they had been assigned to seated wakefulness or a nap opportunity. The study hypothesis and expected direction of effects were not disclosed. Condition labels were not displayed on the performance forms, but contemporaneous documentation sufficient to verify assessor masking was unavailable. Accordingly, the study was not characterized as formally assessor-blinded.
Training, dietary, and stimulant standardization
During the 48 h before each trial, the same coach-planned training content was used across condition days. The morning session remained low to moderate in intensity and included stretching, light jogging, ball handling, technical shooting, and light basketball-specific drills. Additional high-intensity training or strenuous non-prescribed exercise was prohibited during the final 24 h. Because internal and external training-load metrics were not recorded, this procedure was described as schedule and content standardization rather than quantified load matching.
The same cafeteria lunch was provided on every day of the week. The menu was estimated by the cafeteria and a nutrition professional to provide approximately 1,050 kcal, with an approximate energy distribution of 60% carbohydrate, 20% protein, and 20% fat. Food type and portions were kept as similar as practicable, with small body-mass-related adjustments allowed. Minor leftovers were permitted, but additional snacks and caloric beverages were prohibited. From lunch until completion of testing, only water was allowed. Alcohol and caffeine were prohibited during the final 24 h, and coffee and energy drinks were prohibited on the day of the trial. Because individual food intake was not weighed, the menu was reported as an estimate rather than the exact amount ingested.
Recovery-condition timeline and environmental control
All three recovery conditions ended at 14:30 so that the interval from condition completion to warm-up and testing was identical. For N90, athletes were placed in the nap room from 13:00 to 14:30. For N30, supervised seated wakefulness was maintained from 13:00 to 14:00, followed by a nap opportunity from 14:00 to 14:30. During the pre-nap N30 hour, printed reading, quiet conversation, or music was permitted, but recumbency, sleep, and prolonged eye closure were prohibited. For N0, supervised seated wakefulness was maintained from 13:00 to 14:30. Quiet conversation and reading from print were permitted, but lying down, sleep, and prolonged eye closure were prohibited. A researcher remained in the N0 room throughout the condition.
The same darkened, noise-controlled, air-conditioned nap room was used for N30 and N90. The air-conditioning setting was approximately 21 °C; this was a set point or practical estimate rather than a continuously measured temperature, and humidity was not recorded. Four athletes were accommodated per room, with one bed per athlete. Pillows, blankets, eye masks, and earplugs were provided. Mobile phones and personal electronic devices were collected before the nap opportunity. During N30 and N90, music, conversation, food, caffeine, and energy drinks were prohibited.
At 14:30, N30 and N90 participants were awakened using the same low-arousal verbal instruction. A yes/no procedural question was asked regarding whether the athlete believed she had slept; these individual responses were not retained in the archived analysis dataset and did not constitute objective sleep verification. Subjective sleep duration was not estimated because it had not been collected. After N30 and N90 only, perceived sleep quality was obtained using a 100 mm horizontal visual analog scale anchored by 0 mm (“slept very poorly”) and 100 mm (“slept very well”). This scale was not collected after N0. The scale was treated as a subjective descriptor and not as evidence of sleep onset, total sleep time, sleep efficiency, or sleep stage.
Wakefulness buffer, familiarization, warm-up, and fixed test order
Supervised wakefulness was maintained from 14:30 to 15:30 in all conditions. During this buffer, sleep, recumbency, caffeine, energy drinks, additional food, and vigorous unsupervised activity were prohibited. A standardized 15 min group warm-up was conducted from 15:30 to 15:45 using the same lead researcher, verbal instructions, and sequence. The warm-up included light jogging, dynamic stretching, progressive footwork, movements to increase body temperature, and basic basketball-specific actions. An additional individual warm-up was not allowed. Warm-up content and duration were standardized, but intensity was not objectively quantified. The first countermovement-jump trial began approximately 1–2 min after the warm-up.
A full familiarization session was completed 72 h before the first experimental trial. The same venue, equipment, court layouts, test order, start rules, within-test recovery intervals, invalid-trial criteria, and verbal instructions planned for the experimental sessions were used. Familiarization values were not used in the final analysis because they had not been archived for reliability assessment. The test battery was administered in the following fixed order: countermovement jump without arm swing, countermovement jump with arm swing, three-quarter-court sprint, modified 505, Lane Agility Test, and 30–15 Intermittent Fitness Test. This order placed the exhaustive intermittent-running test last1,2,16.
Countermovement-jump assessment
Jump height was measured with a photoelectric jump system; manufacturer and model information were provided in the Table of Materials. The system was checked before every session, and the same hardware and software configuration was used across conditions. For the no-arm-swing countermovement jump, both hands were maintained on the hips, a self-selected countermovement depth was used, take-off and landing were performed with both feet simultaneously, and the knees were kept extended during flight without tucking. For the arm-swing condition, natural arm movement was allowed while simultaneous take-off and landing criteria were retained. Three valid trials per condition were completed with 30 s of passive recovery; invalid attempts were repeated after the prescribed interval, and the highest valid jump height was retained17.
Timing-gate setup and running tests
The same photocell timing-gate system was used for the three-quarter-court sprint, modified 505, and Lane Agility Test; manufacturer and model information were provided in the Table of Materials. The beam height was set to 80 cm, and the gates were verified before every session. For every running test, a stationary start was used, with the lead foot 20 cm behind the start line or the first timing gate, to reduce premature beam interruption. Invalid attempts were recorded, the prescribed recovery was provided, and the trial was repeated.
For the three-quarter-court sprint, the distance from one baseline to the opposite free-throw line was measured as approximately 22.86 m. Two valid trials separated by 45 s were completed, and the fastest time was retained. A false start, premature gate trigger, or start error was treated as invalid.
For the modified 505, the first timing line was placed at the start, the second timing line 10 m from the start, and the turn line 5 m beyond the second timing line. Athletes began with the lead foot 20 cm behind the first line, accelerated through the second line, continued 5 m to the turn line, completed a 180° turn, and returned 5 m through the second line. During familiarization, each athlete was allowed to select a preferred turning direction and plant limb; the same direction and plant limb were required in both trials and all three conditions. The plant foot was required to contact or cross the turn line. Attempts involving failure to reach the line, route error, premature timing activation, or equipment interference were invalidated. Two valid trials separated by 45 s were completed.
The first 10 m split was defined as the interval from the first timing line to the first passage through the second line. The turn/second-10 m segment was defined as the interval from the first to the second passage through the second line, encompassing the 5 m entry, 180° turn, and 5 m return. The combined modified-505 completion time was calculated as the first split plus the turn/second 10 m segment. The turn-segment–approach difference was calculated as the turn/second 10 m segment minus the first split. The physical trial with the fastest valid combined time was selected, and all four modified-505 variables were retained from that same trial. The segment difference was not labeled as a standard change-of-direction deficit because its distances and construction differed from the conventional metric18,19.
For the Lane Agility Test, standard basketball-lane dimensions and the same prescribed movement direction were used for all athletes. Cones were placed at the four corners of the lane. From a stationary start 20 cm behind the gate, athletes completed the sequence of forward sprinting, lateral shuffling, backpedaling, and lateral shuffling around the marked lane, then reversed the route and returned to the finish. Three valid trials separated by 60 s were completed, and the fastest time was retained. Attempts involving apparatus contact, a missed required area, an incorrect movement sequence, or timing interference were invalidated.
30–15 Intermittent fitness test
The 30–15 Intermittent Fitness Test (30–15IFT) was administered on a 28 m shuttle layout using the same audio source, speaker position, court surface, markings, and verbal instructions across conditions. The test began at 10 km·h⁻1 and continued until the athlete voluntarily stopped because of exhaustion or could no longer maintain the prescribed pace and reach the designated zones. Final velocity was recorded as the stopping speed minus 0.5 km·h⁻1, corresponding to the last fully completed stage. This test was administered last16.
Trial retention, data handling, and timing correction
The highest valid trial was retained for each countermovement-jump condition, the fastest valid trial was retained for the three-quarter-court sprint and Lane Agility Test, and the valid modified-505 trial with the fastest combined time was retained. Every modified-505 component was retained from that same selected physical trial. Attempts invalidated for rule violations, false starts, premature gate activation, missed lines or areas, apparatus contact, route error, movement-sequence error, or device interference were excluded. Missing performance values were not estimated, simulated, interpolated, or back-calculated. Ten participant-condition records contained at least one invalid attempt, but a valid repeat was available in each record. Seven participant-condition observations carried a recorded protocol-deviation flag and were retained in the primary analysis; a sensitivity analysis excluded these observations (Table 1).
Retained data were reorganized into one row per participant per condition. The final analysis dataset contained 18 participants, three conditions per participant, 54 participant-condition rows, three participants in each sequence, no missing retained outcomes, and arithmetic agreement among modified-505 variables. Modified-505 derived variables were recalculated from the retained split values of the same selected trial. Native device exports were unavailable for independent source-file verification.
A fixed 0.02 s device-related timing adjustment associated with the SWIFT G4 timing-processing procedure was applied to the N0 turn/second-10 m component. In the dataset, 18 N0 turn/second 10 m values received +0.02 s; the corresponding 18 turn-segment–approach differences and 18 combined times were recalculated, while the first 10 m split values were unchanged. The native SWIFT export and contemporaneous device-level record could not be recovered, so the adjustment could not be independently reverified against the original source file. The corrected and pre-correction datasets were preserved separately, and all nine outcome analyses were repeated with both versions (Table 1).
Statistical analysis
Each of the nine outcomes was analyzed using a linear mixed-effects model with condition, experimental period, and assigned sequence as fixed effects and participant as a random intercept. Models were estimated by restricted maximum likelihood, and Type III fixed-effect tests with Satterthwaite denominator degrees of freedom were obtained using R, lme4, and lmerTest20,21.
Unadjusted means and standard deviations were presented for description. The false-discovery rate across the nine prespecified omnibus condition tests was controlled using the Benjamini–Hochberg procedure22. For outcomes that remained significant after this adjustment, N0 versus N30, N0 versus N90, and N30 versus N90 were compared using estimated marginal means. p values were adjusted using the Holm procedure across the three pairwise contrasts within each outcome23, and Bonferroni-adjusted simultaneous 95% confidence intervals were reported for the same family of three contrasts. Participant-matched Cohen’s dz was calculated from within-participant raw differences24.
Two-sided tests and an adjusted significance threshold of 0.05 were used.
Optimizer messages, singularity, residual-versus-fitted plots, residual Q–Q plots, standardized residuals, and Shapiro–Wilk tests were inspected. Sensitivity analyses were conducted using a period-adjusted mixed model, a condition-only mixed model, a Friedman test with Benjamini–Hochberg correction across the nine outcomes, a mixed model excluding the seven protocol-deviation observations, and a complete corrected-versus-pre-correction rerun. For the paired N30/N90 perceived sleep-quality ratings, a paired t-test and a Wilcoxon signed-rank sensitivity analysis using the normal approximation with continuity correction were used. Diagnostic disagreement was treated as a reason for cautious interpretation rather than as a basis for selecting the most favorable analysis. The final analysis code, software environment, analysis datasets, diagnostic output, and figure-source data were archived in the repository.