Aggregate Compaction Assay
Aggregate Compaction Assay - Related Videos
09:22
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
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Cited by 21 •
2015
Unmodified and hyperphosphorylated tau proteins were used in two in vitro aggregation assays to reveal the hyperphosphorylation-dependent fast aggregation kinetics. These assays pave the way for future screens for compounds that can modulate the propensity of hyperphosphorylated tau to form fibrils that underlie the progression of Alzheimer’s disease.
01:27
Compact Bone
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2025
Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function. Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
04:12
Protein Aggregate Formation Assay: A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor
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2025
In this video, we demonstrate a cell-based protein aggregation assay using proteasome inhibitors, which block proteasome activity, preventing misfolded, mutant proteins, fused to a fluorescent label, from undergoing ubiquitin-dependent proteasomal degradation, leading to their accumulation within the cell cytoplasm. The protein aggregates are then visualized and quantified by fluorescence microscopy.
04:32
PolyQ Aggregation Assay to Identify Neuroprotective Effect of a Compound in Worms
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2025
This video describes an in vivo method to screen the protective potential of neuroprotective test compounds in reducing polyQ aggregation in body wall muscle cells of nematodes and ameliorating aggregation-associated proteotoxicity.
04:16
Cell Aggregation Assay to Detect Trans Interactions between Cell Adhesion Molecules
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2025
In this video, we demonstrate the technique to measure transcellular interactions between synaptic cell adhesion molecules through the HEK cell aggregation assay. In this assay, an equal number of HEK cells expressing a desired presynaptic cell adhesion molecule and a fluorescent protein are mixed with HEK cells expressing a post-synaptic cell adhesion molecule and another fluorescent protein. The interaction between the cell adhesion molecules forces the cells to aggregate, which can be...
FAQs
As cells establish more cohesive cell-cell contacts, the aggregate can rearrange and contract, producing a measurable reduction in area or diameter. This makes the assay useful for linking a visible geometric change to altered adhesion and tissue organization. In bioengineering, the same readout can indicate how an engineered construct assembles under different culture conditions.
Greater reductions in aggregate area or diameter generally indicate stronger compaction, but the measurement is not independent of biological context. Cell type and culture conditions can affect how aggregates change shape or size. Therefore, compaction values are most informative when researchers compare samples under defined conditions and interpret geometric changes alongside the relevant experimental treatment.
Time-dependent tracking captures the progression of cellular rearrangement, contraction, and cohesive contact formation. This provides more information about how an aggregate organizes than a single observation would provide. Monitoring the trajectory of area or diameter can help distinguish gradual assembly behavior from a limited change in packing, supporting comparisons between tissue-forming strategies.
Cells are first assembled into aggregates under nonadherent conditions, which supports cluster formation rather than attachment to a surface. Researchers then track aggregate shape or size as the cells rearrange and contract. Reductions in measured area or diameter provide the quantitative compaction readout, while consistent cell type and culture conditions improve comparisons between experimental groups.
Researchers can measure how aggregates respond after exposure to different materials or treatments, or after using distinct scaffold-free assembly strategies. Comparing changes in area or diameter identifies conditions associated with greater or lesser compaction. These results help evaluate cell-cell interactions and determine how a selected strategy affects the organization and behavior of engineered tissue constructs.
Compaction measurements connect aggregate geometry with broader features of engineered tissue formation, including cell-cell interactions, tissue organization, and aggregate mechanics. When related to functional performance, the assay can help researchers judge whether a construct assembles cohesively and whether its organization changes under a selected engineering approach. This supports evaluation of scaffold-free tissue formation and construct design.