Yeast Metabolic Network
Yeast Metabolic Network - Related Videos
08:30
Yeast Maintenance
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2023
Research performed in the yeast Saccharomyces cerevisiae has significantly improved our understanding of important cellular phenomona such as regulation of the cell cycle, aging, and cell death. The many benefits of working with S. cerevisiae include the facts that they are inexpensive to grow in the lab and that many ready-to-use strains are now commercially available. Nevertheless, proper maintenance of this organism is critical for successful experiments. This video will provide an overview...
07:48
Yeast Reproduction
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2023
Saccharomyces cerevisiae is a species of yeast that is an extremely valuable model organism. Importantly, S. cerevisiae is a unicellular eukaryote that undergoes many of the same biological processes as humans. This video provides an introduction to the yeast cell cycle, and explains how S. cerevisiae reproduces both asexually and sexually Yeast reproduce asexually through a process known as budding. In contrast, yeast sometimes participate in sexual reproduction, which is important because...
05:20
Yeast Two-Hybrid Assay to Determine Protein Self-Association in Yeast Cells
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2025
This video demonstrates the yeast two-hybrid assay to detect specific protein self-interactions. In this assay, the protein of interest is tagged to two domains of a transcription factor that encodes the β-galactosidase enzyme. When the two proteins interact, this causes transcription and translation to form the enzyme, which is then detected using a biochemical assay.
08:30
Yeast Transformation and Cloning
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2023
S. cerevisiae are unicellular eukaryotes that are a commonly-used model organism in biological research. In the course of their work, yeast researchers rely upon the fundamental technique of transformation (the uptake of foreign DNA by the cell) to control gene expression, induce genetic deletions, express recombinant proteins, and label subcellular structures. This video provides an overview of how and why yeast transformation is carried out in the lab. The important features of yeast...
06:53
In Vivo Monitoring of Transcriptional Activity During Metabolic Transition Using a Bioluminescent Reporter in Yeast
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2025
This protocol employs a bioluminescent reporter, allowing measurements of transcriptional activity in Saccharomyces eubayanus to monitor the glucose-to-maltose transition, enabling real-time analysis of metabolic adaptations and supporting strain optimization for industrial fermentation under diverse conditions.
FAQs
Metabolic flux shifts as enzymes, cofactors, nutrient conditions, and regulatory interactions alter the relative activity of competing reactions. Carbon can therefore be directed toward cellular energy, biomass formation, or specialized products rather than following one fixed route. Tracking these changes helps bioengineers identify where pathway activity limits production or diverts resources away from a desired compound.
Cofactors support enzyme-driven reactions, while nutrient conditions influence which pathways receive metabolic activity. Changes in either can modify the balance between energy generation, biosynthesis, fermentation, and respiration. In engineering studies, accounting for these variables helps explain altered flux patterns and supports designs that maintain sufficient growth while directing more carbon toward a target product.
Gene regulation changes the amount or activity of components that control biochemical reactions and transport processes. This can strengthen one route while reducing another, reshaping the distribution of metabolites through the cell. Understanding that regulation is important when predicting cellular responses to genetic changes, because an intervention may affect growth, energy production, and product formation simultaneously.
Fermentation and respiration represent different pathway options within the broader metabolic system, and their relative activity influences how nutrients support energy generation and biosynthesis. Comparing these routes allows researchers to examine where carbon and metabolic activity are being directed. Such analysis is useful when redesigning cells for improved growth or for production of fuels and other specialized compounds.
Researchers combine network models with experimental measurements to examine reactions, transport processes, regulatory interactions, and metabolic flux. The analysis can reveal bottlenecks, competing routes, and imbalances between growth and product formation. These findings provide a basis for proposing genetic or pathway changes and for predicting how the cell may respond before testing a redesigned system.
Together, measurements and models can indicate how nutrients are distributed among energy generation, biomass formation, and specialized-product pathways. They can also identify pathway bottlenecks and estimate how genetic changes may alter cellular behavior. This information guides efforts to improve production of fuels, pharmaceuticals, enzymes, or other valuable compounds while supporting more efficient and stable microbial systems.