Endotracheal Instillation
Endotracheal Instillation - Related Videos
06:58
Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope
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Cited by 12 •
2014
We have developed a simple, reliable, and relatively inexpensive method for endotracheal intubation in mice via direct laryngoscopy using an otoscope with a 2.0 mm speculum. This technique is atraumatic and can be used for repeated measurements in chronic experiments. We find it superior to tracheostomy or previously reported nonsurgical techniques.
04:50
Direct Tracheal Instillation of Solutes into Mouse Lung
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Cited by 54 •
2010
Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice.
06:57
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Cited by 48 •
2015
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
04:46
Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models
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2025
This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.
05:40
Establishment of A Mouse Sepsis-Induced Immunosuppression Model By Intranasal Instillation of Bacteria
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2026
Here, we present a protocol for establishing a mouse sepsis immunosuppression model via intranasal instillation of Klebsiella pneumoniae and validated the model through various indicators.
FAQs
After introduction through the endotracheal tube, airflow and ventilation carry the material through the lower respiratory tract. This movement allows the substance to reach bronchial and alveolar spaces, where its distribution can be examined. Because ventilation participates in transport, the resulting exposure reflects both the introduced material and how it is conveyed through the respiratory system.
Localized exposure focuses the test substance on the lower respiratory tract rather than distributing it broadly throughout the body. This supports more direct investigation of pulmonary responses and helps researchers relate observed biological effects to the material delivered into the lung. The approach is therefore useful when the primary research question concerns respiratory tissues or lung-specific effects.
Researchers can assess both where the introduced material distributes and how the lung responds biologically. Relevant outcomes include pulmonary drug effects, respiratory disease-related responses, toxicological effects, and inflammation. Examining bronchial and alveolar spaces connects the exposure site with measurable changes in the lower respiratory tract, supporting controlled studies of lung biology.
The core workflow consists of introducing a liquid, suspension, or other test substance through an endotracheal tube, allowing it to enter the trachea, and relying on airflow and ventilation to carry it into lower respiratory regions. Researchers then assess distribution and biological effects. These stages provide a controlled route from administration to evaluation of pulmonary responses.
This technique is particularly relevant when a study requires controlled delivery to the lower respiratory tract. Biology researchers may apply it in investigations of pulmonary drug delivery, respiratory disease, toxicology, or lung inflammation. Its localized exposure and reproducible investigation of lung responses make it suitable for comparing how test substances affect pulmonary tissues.
In experimental biology, the method supports evaluation of substance distribution and lung effects under controlled exposure conditions. In clinical contexts, endotracheal delivery can administer treatments such as pulmonary surfactant. Together, these uses show how the same access route can support both mechanistic respiratory research and treatment delivery directed toward the lower respiratory tract.