Right Ventricular Puncture

Right ventricular puncture is a technique that uses a needle to access the heart’s right ventricle, enabling direct sampling of cardiac blood or measurement of ventricular pressure in biological research. The procedure relies on anatomical localization of the right ventricle and controlled needle placement through the ventricular wall, followed by aspiration or connection to a pressure-monitoring system. Researchers use it to evaluate cardiac function, investigate pulmonary circulation and right-heart disease, and collect blood when peripheral sampling is impractical. When performed with appropriate controls and tissue-handling procedures, right ventricular puncture provides data that support studies of cardiovascular physiology, pathology, and experimental treatments.

Right Ventricular Puncture - Related Videos

Research

JoVE Journal - Medicine

Cecal Ligation Puncture Procedure

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Cited by 200 •

2011

The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.

Cardiac Blood Collection via Subclavian Puncture in Mice

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2025

Subclavian cardiac puncture is a simple, reliable method for collecting terminal blood in mice. It is easy to operate and ensures high-purity samples.

Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture

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Cited by 87 •

2007

Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.

Use of Two Intracorporeal Ventricular Assist Devices As a Total Artificial Heart

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Cited by 4 •

2018

Here, we present a protocol using two centrifugal pumps as a total artificial heart replacement.

Measuring Left Ventricular Pressure in Late Embryonic and Neonatal Mice

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Cited by 18 •

2012

Measuring left ventricular pressure (LV) in embryonic and neonatal mice is described. Pressure is measured by inserting a needle connected to a fluid-filled transducer into the LV under ultrasound guidance. Care must be taken to maintain normal cardiac function during the experimental protocol.

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