Extracellular Fluid Isolation
Extracellular Fluid Isolation - Related Videos
08:33
Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans
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Cited by 4 •
2012
The model organism C. elegans uses pseudocoelomic fluid as a passive circulatory system. Direct assay of this fluid has not been previously possible. Here we present a novel technique to directly assay the extracellular space, and use systemic silencing signals during an RNAi response as a proof of principle example.
02:29
Microdialysis of Interstitial Fluid from a Mouse Brain to Isolate Extracellular Proteins
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2025
The video demonstrates the process of using a microdialysis probe to collect extracellular proteins from the interstitial fluid (ISF) of a mouse brain. A microdialysis probe, equipped with a high-molecular-weight cut-off semi-permeable membrane, is inserted into the brain of an anesthetized mouse via an implanted guide cannula. The probe, connected to pumps, allows a physiological buffer to perfuse the brain tissue and mix with the ISF. Proteins in the ISF diffuse across the semi-permeable...
03:37
Visualization of Extracellular Traps in Macrophages from Human Bronchoalveolar Lavage Fluids
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2025
This video demonstrates the immunolabeling of macrophage extracellular traps, METs, in bronchoalveolar macrophages from bronchoalveolar lavage fluid, and their visualization using a confocal microscope.
09:18
Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique
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Cited by 22 •
2018
Here, we describe two extracellular vesicle isolation protocols, ultrafiltration centrifugation and ultracentrifugation with density gradient centrifugation, to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized.
05:07
Characterizing Extracellular Vesicles from Biological Fluids
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Cited by 3 •
2025
Here, we demonstrate the characterization method for extracellular vesicles (EVs) collected from biological fluids, such as tears and saliva, of human subjects. The scanner used in this method is capable of detecting the phenotype, size, and total particle count of EVs from 1 µL of the sample.
FAQs
Contamination can introduce cellular material into the fluid fraction and obscure its native composition. Careful collection helps ensure that measured proteins, metabolites, ions, and signaling molecules originate primarily from the extracellular environment rather than from disrupted cells or unrelated debris. This improves the connection between fluid composition and the physiological state being investigated.
Both approaches clarify the sample by removing cells and larger debris while retaining soluble components in the fluid. Filtration separates material according to passage through a filter, whereas differential centrifugation uses sequential separation of particulate material. The resulting clarified fraction is therefore better suited for examining dissolved molecular constituents without the bulk of cellular material.
The isolated fraction can be examined for dissolved proteins, metabolites, ions, and signaling molecules. Together, these components provide information about the local cellular environment, including communication between cells and aspects of tissue homeostasis. Comparing their composition with physiological state can also reveal changes associated with disease or treatment responses.
Because signaling molecules are present outside cells, analyzing the clarified fluid can provide direct information about extracellular communication signals. Researchers can relate these molecules to the surrounding tissue environment and to changes in physiological state. This makes the method useful for investigating how cellular activity is reflected in the composition of nearby biological fluid.
A typical workflow begins with biological sample collection under conditions that reduce contamination. The sample is then clarified by filtration or differential centrifugation to remove cells and debris. The remaining fluid fraction contains soluble material for analysis, allowing researchers to characterize its molecular composition and relate the findings to tissue or cellular status.
Researchers apply this approach when they need to study the extracellular environment rather than cellular material itself. It supports investigations of cell communication, tissue homeostasis, disease-related changes, and responses to treatment. The resulting measurements can help characterize biological microenvironments and identify measurable biomarkers relevant to basic and translational research.
Changes in the concentrations or composition of extracellular proteins, metabolites, ions, and signaling molecules may reflect how a biological system responds to treatment. By examining these soluble components after clarification, researchers can compare extracellular states and identify measurable molecular changes associated with treatment effects, while keeping the analysis focused on the surrounding fluid environment.