Vampiric Isolation of Extracellular Fluid from <em>Caenorhabditis elegans</em>

Stephen A. Banse; Craig P. Hunter

doi:10.3791/3647

Journal

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Biology

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Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans

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Biology

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JoVE Journal Biology

Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans

Vampiric Isolation of Extracellular Fluid from Caenorhabditis elegans

10,083 Views

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08:33 min

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March 19, 2012

DOI:

10.3791/3647-v

DOI:

10.3791/3647-v

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08:33 min

March 19, 2012

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2 Views

Stephen A. Banse¹, Craig P. Hunter¹

¹Department of Molecular and Cellular Biology,Harvard University

Summary

The model organism C. elegans uses pseudocoelomic fluid as a passive circulatory system. Direct assay of this fluid has not been previously possible. Here we present a novel technique to directly assay the extracellular space, and use systemic silencing signals during an RNAi response as a proof of principle example.