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Chapters
- 00:05Title
- 01:13Preparation of Donor Worms
- 03:08Preparation of Recipient Worms
- 03:49Vampiric Isolation of Extracellular Fluid
- 06:59Representative RNAi Results
- 08:12Conclusion
The model organism C. elegans uses pseudocoelomic fluid as a passive circulatory system. Direct assay of this fluid has not been previously possible. Here we present a novel technique to directly assay the extracellular space, and use systemic silencing signals during an RNAi response as a proof of principle example.
