Fourier Transform Mass Spectrometry

Fourier Transform Mass Spectrometry (FTMS) is a high-resolution analytical method that determines the mass-to-charge ratios of ions, enabling precise characterization of complex biological molecules. In an FTMS instrument, ions are trapped in an electromagnetic field, where their motion produces an image current; a Fourier transform converts the measured frequencies into a mass spectrum based on each ion’s mass-to-charge ratio. The technique provides exceptional mass resolving power and mass accuracy for analyzing proteins, peptides, metabolites, lipids, and other biomolecules. In biology, FTMS supports proteomics, metabolomics, structural analysis, and the detection of subtle molecular changes in cells, tissues, and biological fluids.

Fourier Transform Mass Spectrometry - Related Videos

Research

JoVE Journal - Engineering

A Multimodal Wide-Field Fourier-Transform Raman Microscope

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2025

A wide-field Fourier-transform microscope, based on a compact and ultra-stable birefringent interferometer, allows the parallel acquisition of spectra for all pixels of a 2D detector. The time-domain approach enables the disentanglement of photoluminescence and Raman signals, and allows rapid Raman mapping (~5 ms/pixel) with ~1-µm spatial and 23-cm-1 spectral resolution.

Education

JoVE Core - Electrical Engineering

Fast Fourier Transform

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2024

The Fast Fourier Transform (FFT) is a computational algorithm designed to compute the Discrete Fourier Transform (DFT) efficiently. By breaking down the calculations into smaller, manageable sections, the FFT significantly reduces the computational complexity involved. Direct computation of an N-point DFT requires N2 complex multiplications, whereas the FFT algorithm needs only (N/2)log⁡2N multiplications, offering a much faster performance. The computational efficiency of the FFT becomes...

A Fourier Transform Infrared Spectroscopy Technique to Study Peptide Self-Assembly

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2025

This video demonstrates the self-assembly of amyloid peptides into a beta sheet-rich supramolecular structure using Fourier transform infrared spectroscopy. An infrared beam is passed through a crystal with a high refractive index, and energy absorption across the interface of the crystal and the sample is recorded. The secondary structures of the sample proteins are identified by analyzing their characteristic absorption peaks at specific regions of the infrared spectrum, which aids in...

Properties of Fourier Transform I

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2024

The application of Fourier Transform properties in radio broadcasting is multifaceted, enabling significant advancements in the way signals are transmitted and received. Key areas where these properties are utilized include simultaneous multi-channel transmission, audio clip speed adjustments, live broadcast delays for different time zones, audio frequency adjustments, and signal demodulation. In radio broadcasting, multiple audio signals often need to be transmitted simultaneously. The Fourier...

Properties of Fourier Transform II

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2024

The Fourier Transform (FT) is an essential mathematical tool in signal processing, transforming a time-domain signal into its frequency-domain representation. This transformation elucidates the relationship between time and frequency domains through several properties, each revealing unique aspects of signal behavior. The Frequency Shifting property of Fourier Transforms highlights that a shift in the frequency domain corresponds to a phase shift in the time domain. Mathematically, if x(t) has...

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