Klett Colorimeter
Klett Colorimeter - Related Videos
02:45
Culturing Neisseria gonorrhoeae in Metal-Depleted Conditions
0 Views •
2025
Source: Maurakis, S., and Cornelissen, C. N. Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands. J. Vis. Exp. (2020)This video demonstrates the procedure for growing Neisseria gonorrhoeae under metal-limited conditions using a defined, metal-depleted medium.
10:48
Nutrients in Aquatic Ecosystems
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Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University Nitrogen and phosphorus are essential plant nutrients found in aquatic ecosystems and both are monitored as a part of water quality testing because in excess amounts they can cause significant water quality problems. Nitrogen in water is measured as the common form nitrate (NO3-) that is dissolved in water and readily absorbed by photosynthesizers such as algae. The common form of phosphorus measured is phosphate...
09:46
Synthesis of Plant Phenol-derived Polymeric Dyes for Direct or Mordant-based Hair Dyeing
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Cited by 12 •
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Here, we present a protocol to use pre-synthesized polymeric products derived from fungal laccase-catalyzed polymerization of plant phenols, either with or without mordant agents (e.g., FeSO4), to induce detergent-resistant keratin hair dyeing within 2.5 hours.
12:37
Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
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Cited by 13 •
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Epidermal melanin is induced by topical application of forskolin in a murine model of the fair-skinned UV-sensitive human. Pharmacologic manipulation of cAMP levels in the skin and epidermal darkening strongly protect against UV-mediated inflammation (sunburn) as measured by the minimum erythematous dose (MED) assay.
FAQs
The filter determines which portion of the light reaches the sample and photocell. This makes the measurement responsive to the color or optical behavior of the material under examination. Choosing a filter appropriate to the sample helps researchers compare readings consistently, particularly when tracking changes in pigmentation or a biochemical reaction over time.
Suspended cells and particles scatter light passing through the liquid, reducing the amount that reaches the detector. As the suspension becomes more turbid, the instrument generally records a higher Klett value. This relationship allows changes in cloudiness to serve as an indirect indicator of changing cell density or particle concentration in a biological sample.
Colored substances primarily affect the measurement by absorbing selected light, whereas suspended particles influence it mainly by scattering light. Both effects alter the detector response, but they represent different sample properties. Interpreting a reading therefore requires knowing whether the experiment is following pigmentation or biochemical color change, rather than assuming every value reflects suspended-cell abundance.
A typical measurement directs light through the selected filter and the liquid sample, after which a photocell detects the transmitted light and the instrument displays a Klett reading. Researchers can repeat this process for samples collected at different stages or conditions, then compare the values to evaluate changes in turbidity, pigmentation, or reaction state.
It is useful when researchers need rapid comparisons of microbial culture growth or estimated cell density. Repeated readings can show whether a culture becomes more turbid as growth changes, supporting quantitative monitoring without requiring highly precise spectrophotometric equipment. The approach is especially practical when the main goal is to follow relative changes among samples or time points.
Researchers can compare changes in microbial turbidity, pigmentation, and biochemical reactions by recording Klett values under consistent measurement conditions. The resulting readings provide quantitative comparisons between samples or stages of an experiment. In biology, this supports studies that need a straightforward estimate of optical change rather than a detailed analysis requiring more precise spectrophotometric instrumentation.