Tx-114 Surfactant
Tx-114 Surfactant - Related Videos
09:34
Extraction and Characterization of Surfactants from Atmospheric Aerosols
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Cited by 26 •
2017
Methods are presented for the targeted extraction of surfactants present in atmospheric aerosols and the determination of their absolute concentrations and surface tension curves in water, including their Critical Micelle Concentration (CMC).
16:11
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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Cited by 380 •
2013
This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and process data.
07:20
Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS)
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Cited by 21 •
2016
Repeated pulmonary lavages in anesthetized pigs induce lung injury resembling major aspects of human acute respiratory distress syndrome (ARDS). For this purpose the lungs are repeatedly lavaged with 0.9% saline at 37 °C. The goal of the protocol is a reproducible mitigation of gas exchange and hemodynamics for research in ARDS.
10:28
Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
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Cited by 15 •
2014
This paper demonstrates the experimental procedure to measure terminal settling velocities of spherical particles in surfactant-based shear thinning viscoelastic fluids. Fluids over a wide range of rheological properties are prepared and settling velocities are measured for a range of particle sizes in unbounded fluids and fluids between parallel walls.
04:16
Enrichment of Bacterial Lipoproteins Using Non-Ionic Detergent Phase Separation
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2025
Source: Armbruster, K. M., Meredith, T. C. Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry. J. Vis. Exp. (2018)This video demonstrates the enrichment of bacterial lipoproteins using a non-ionic detergent that undergoes phase separation upon warming. Through sequential temperature shifts and centrifugation steps, lipoproteins are selectively partitioned into the detergent phase, while non-lipoprotein contaminants...
FAQs
Temperature controls the key separation event. When the solution is heated above the surfactant’s cloud point, it divides into a detergent-rich phase and a detergent-poor aqueous phase. Hydrophobic proteins, lipids, and membrane fragments can then partition selectively between these phases. This behavior converts differences in membrane association into separable biochemical fractions for downstream analysis.
The nonionic character of Tx-114 supports its use in solubilizing membrane-associated molecules, while the phase-separation step can preserve protein complexes under relatively mild conditions. This makes the surfactant useful when a preparation must both release membrane proteins and retain biochemical associations for subsequent study. In biology, that balance helps connect extraction with membrane composition and protein localization.
Selective partitioning allows researchers to distinguish detergent-soluble membrane material from components that remain in the detergent-poor phase. Hydrophobic proteins, lipids, and membrane fragments are the relevant classes identified in the source, so the resulting distribution can help separate membrane-associated material from other cellular components. This provides a biochemical basis for examining membrane composition and protein localization.
A basic workflow begins by exposing a biological sample to Tx-114 so membrane-associated molecules and membrane fragments become solubilized. The preparation is then brought above the cloud point, where it separates into detergent-rich and detergent-poor phases. Researchers can use the resulting partitioning to obtain fractions enriched for selected membrane-associated material or to remove detergent-soluble contaminants.
Researchers choose this approach when they need membrane protein extraction, biochemical fractionation, or removal of lipids and detergent-soluble contaminants. It is particularly relevant for samples in which membrane-associated molecules must be separated from other cellular components. Because the process may preserve protein complexes under relatively mild conditions, it can support analyses that depend on maintaining those associations.
Tx-114-based fractionation can provide information about where molecules reside within a cellular preparation. Differences in phase distribution help researchers assess membrane composition and protein localization, while removal of lipids or detergent-soluble contaminants can simplify biochemical samples. In this way, the technique connects physical separation with studies of cellular biochemistry rather than serving only as an extraction step.