Pressure-assisted Delivery
Pressure-assisted Delivery - Related Videos
02:51
An Ultrasound-Assisted Delivery of Antibodies in a Mouse Model
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2025
This video demonstrates a procedure of ultrasound-assisted antibody delivery in a mouse model. Retro-orbitally injected microbubbles oscillate under ultrasound, creating a transient disruption in the blood-brain barrier, permitting labeled antibodies to enter the brain.
10:51
Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
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Cited by 2 •
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A microfluidic vortex assisted electroporation platform was developed for sequential delivery of multiple molecules into identical cell populations with precise and independent dosage control. The system’s size based target cell purification step preceding electroporation aided to enhance molecular delivery efficiency and processed cell viability.
03:31
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
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2025
In this video, we demonstrate the laser-assisted permeation of the protective layer of zona pellucida in mouse fertilized eggs for facilitating lentiviral gene delivery. Lentivirus enables the generation of transgenic animals with a gene of interest stably integrated into their genome.
07:26
Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Cited by 5 •
2013
The laser heated diamond anvil cell combined with synchrotron micro-diffraction techniques allows researchers to explore the nature and properties of new phases of matter at extreme pressure and temperature (PT) conditions. Heterogeneous samples can be characterized in situ under high pressure by 2D mapping and combined powder, single-crystal and multigrain diffraction approaches.
06:03
Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
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Cited by 2 •
2018
Mouse fertilized eggs and early stage embryos are protected by the zona pellucida, a glycoprotein matrix that forms a barrier against gene delivery. This article describes a protocol for perforating the zona with a laser to transduce embryonic cells with lentiviral vectors and to create transgenic mice.
FAQs
The pressure gradient provides the driving force that moves a cargo-containing fluid across a narrow interface, membrane, or tissue barrier. Its effect is evaluated together with exposure time, cargo properties, and the physical structure of the target. These variables determine how effectively the material crosses the barrier and help researchers regulate where and how much cargo is delivered.
Cargo properties influence how the material moves with the applied fluid, while the target’s physical structure determines the barriers that must be crossed. A membrane, tissue, or other biological structure can therefore change the resulting distribution. Considering both factors allows investigators to interpret differences in delivery and adjust the process for more controlled biological experiments.
Passive diffusion relies on spontaneous movement, whereas pressure-assisted delivery adds an externally applied driving force. This distinction becomes important when diffusion alone does not transport sufficient material across a biological barrier. By using controlled pressure and exposure conditions, investigators can support delivery of experimental cargoes in situations where passive movement would be inadequate or less predictable.
Pressure, exposure time, cargo properties, and the physical structure of the target are central control variables. Pressure supplies the driving force, exposure time affects contact with the barrier, and cargo characteristics influence transport. The target’s structure determines the pathway and resistance encountered. Together, these factors shape delivery efficiency, distribution, and dose control.
A typical approach places the selected cargo in a fluid, positions it against the relevant membrane, narrow interface, or tissue barrier, and applies pressure for a defined exposure period. Researchers then evaluate the resulting delivery in the target system. The workflow is organized around controlling the pressure conditions, cargo, contact site, and exposure time.
Researchers may choose pressure-assisted delivery when passive diffusion is insufficient for moving a substance into cells, tissues, or another biological system. The method is useful when experiments require more controlled transport across a physical barrier. Its pressure-driven and spatially targeted features can support studies involving nucleic acids, proteins, drugs, or experimental reagents.
The approach can introduce nucleic acids, proteins, drugs, and experimental reagents for genetic manipulation, imaging, and functional studies. These applications allow investigators to examine how biological systems respond when selected materials reach defined targets. In broader research and development contexts, the same control over delivery can inform laboratory strategies and the development of therapeutic approaches.