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Biology

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Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs
 

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Article doi: 10.3791/58327
November 1st, 2018

Summary November 1st, 2018

Mouse fertilized eggs and early stage embryos are protected by the zona pellucida, a glycoprotein matrix that forms a barrier against gene delivery. This article describes a protocol for perforating the zona with a laser to transduce embryonic cells with lentiviral vectors and to create transgenic mice.

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