Proximal Tubule Transduction
Proximal Tubule Transduction - Related Videos
07:06
Isolation of Primary Human Proximal Tubule Epithelial Cells and Their Use in Creating a Microphysiological Model of the Renal Proximal Tubule
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Cited by 3 •
2025
We present an optimized protocol for processing whole human kidneys to isolate and culture primary renal proximal tubule epithelial cells and the application of these cells in a three-dimensional, microfluidic, microphysiological platform to recapitulate the renal proximal tubule.
05:11
Malpighian Tubule Dissection from an Adult Fly: Isolating an Osmoregulatory and Excretory Organ
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2023
This article describes the dissection of the anterior Malpighian tubules (MT) from a female Drosophila fly and an example protocol from Rossano et al. (2017) in which the MTs are prepared for live imaging in a perfusion chamber.
04:25
Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules
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2025
This video demonstrates the isolation procedure of Sertoli cells from mouse seminiferous tubules, a component of the testes. Enzymatic digestion and repeated washes promote the release of Sertoli cells into the solution, which can be cultured further for proliferation and growth.
11:54
Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator
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Cited by 9 •
2017
Cellular ion transport can often be assessed by monitoring intracellular pH (pHi). Genetically Encoded pH-Indicators (GEpHIs) provide optical quantification of intracellular pH in intact cells. This protocol details the quantification of intracellular pH through cellular ex vivo live-imaging of Malpighian tubules of Drosophila melanogaster with pHerry, a pseudo-ratiometric genetically encoded pH-indicator.
01:16
Transduction
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2025
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...
FAQs
The introduced DNA or RNA can direct production of a target protein or modify pathways within proximal tubule cells. Those changes provide a controlled way to examine effects on solute transport, metabolism, or responses to injury. Linking the genetic intervention to altered cell behavior helps investigators study how specific molecular factors contribute to proximal tubule function.
This kidney region has specialized roles in solute transport and metabolism, making it a relevant setting for testing genes that influence these processes. Its cells can also be examined for injury responses, so genetic manipulation may connect molecular changes with functional or damage-related outcomes. The approach therefore links gene expression to biologically important renal activities.
Cultured proximal tubule cells allow investigators to examine a genetic change in a more controlled cellular setting than a whole-animal study. This can make relationships between an introduced sequence and cell pathways easier to analyze while reducing reliance on animal experiments. Engineered kidney models provide another way to investigate selected mechanisms before broader biological evaluation.
A typical workflow begins by selecting genetic material intended to produce a target protein or alter a cellular pathway. A viral vector then delivers the DNA or RNA into cultured proximal tubule cells. Investigators can examine the resulting changes in gene expression and relate them to solute transport, metabolism, or injury responses.
The method supports analysis of how altered gene expression affects proximal tubule physiology, including pathways associated with solute transport and metabolism. It can also be used to study cellular responses to injury and mechanisms relevant to renal disease. These outcomes help connect candidate genes with specific aspects of kidney-cell behavior.
Researchers can use genetically modified proximal tubule cells to evaluate candidate genes, investigate molecular treatments, and examine responses relevant to nephrotoxicity. Because the cells represent a specialized kidney region, the system can help assess how molecular changes influence renal processes. Engineered kidney models extend these applications while supporting studies that limit dependence on whole-animal experiments.