Tissue Core Harvesting
Tissue Core Harvesting - Related Videos
12:29
Sterile Tissue Harvest
0 Views •
2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Cited by 53 •
2016
This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.
06:06
Apparatus for Harvesting Tissue Microcolumns
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Cited by 7 •
2018
Here we describe a protocol for producing harvesting needles that can be used to collect full-thickness skin tissue without causing donor site scarring. The needles can be combined with a simple collection system to achieve high-volume harvesting.
09:51
A Polymer-based Piezoelectric Vibration Energy Harvester with a 3D Meshed-Core Structure
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Cited by 4 •
2019
In this study, we fabricated a flexible 3D mesh structure and applied it to the elastic layer of a bimorph cantilever-type vibration energy harvester for the purpose of lowering resonance frequency and increasing output power.
FAQs
Orientation preserves the relationship between the sampled region and surrounding tissue architecture, while depth control determines which cellular layers or internal zones enter the core. If either is inconsistent, cores from different specimens may represent non-equivalent locations, weakening comparisons. Careful positioning therefore supports reliable links between microscopic structure and molecular or cellular measurements.
Clean handling helps limit contamination that could interfere with histology, immunostaining, or molecular assays performed on the harvested material. Maintaining the core intact also reduces loss of spatial information within the sampled region. These precautions are especially important when multiple defined regions or specimens will be compared, because contamination or disruption can complicate interpretation.
The technique allows researchers to remove selected regions rather than analyze an entire specimen as one undifferentiated sample. Cores can then be compared across locations, specimens, developmental states, disease conditions, or treatment groups. This focused sampling connects regional tissue structure with cellular or molecular changes while conserving more of the original specimen for additional analyses.
A hollow punch or coring tool is positioned over the selected tissue region, advanced to the required depth, and withdrawn with the core intact. The sample should remain correctly oriented and handled cleanly after removal. These steps preserve the intended spatial context and produce material suitable for downstream biological analysis.
Harvested cores can support histology, which examines tissue structure, and immunostaining, which assesses the distribution of selected biological features within that structure. They can also be used for molecular assays and tissue microarray construction. The appropriate analysis depends on the research question, but each approach benefits from sampling a defined, traceable region.
Biologists use this sampling approach when they need focused, comparable material from valuable tissue specimens. It is relevant to studies of development, disease, and experimental treatments, where regional structural changes may need to be related to cellular or molecular measurements. Tissue microarray construction also enables defined cores from specimens to be organized for comparative analysis.