Wide-field Fluorescence Microscopy
Wide-field Fluorescence Microscopy - Related Videos
06:42
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Cited by 32 •
2013
We review our recent results on the integration of fluorescent microscopy and imaging flow cytometry tools on a cell-phone using compact and cost-effective opto-fluidic attachments. These cell-phone based micro-analysis devices might be useful for cytometric analysis, such as performing various cell counting tasks as well as for high-throughput screening of e.g., water samples in resource limited settings.
09:22
Introduction to Fluorescence Microscopy
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2023
Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently...
10:33
Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
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Cited by 8 •
2017
In the heart, molecular events coordinate the electrical and contractile function of the organ. A set of local field fluorescence microscopy techniques presented here enables the recording of cellular variables in intact hearts. Identifying mechanisms defining the cardiac function is critical in understanding how the heart works under pathological situations.
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
FAQs
Selected wavelengths excite the fluorophores attached to structures of interest, while optical filters reject much of the incoming excitation light. The remaining emitted fluorescence reaches the camera and forms the recorded image. This separation helps preserve the signal from labeled structures, allowing them to be visualized within the illuminated specimen.
Fluorescent labels connect the structures being examined to visible image signals. In cancer studies, they can mark molecular indicators, proliferating cells, tumor-cell features, or cellular relationships within tissue samples. Choosing labels that correspond to the biological question allows the recorded fluorescence to be interpreted in relation to morphology, biomarker expression, proliferation, or tissue-level interactions.
The broad illumination area makes the method useful when a study must inspect a relatively large tissue region quickly. Its straightforward workflow supports screening, comparative analysis, and documentation of experimental responses. In cancer research, these capabilities help investigators examine patterns across cells or tissue samples without limiting observation to a narrowly selected structure.
A basic imaging workflow begins with a specimen containing fluorescently labeled structures, followed by selection of excitation wavelengths for the labels. The specimen is illuminated across the field, emitted light passes through filters that reject much of the excitation light, and a camera records the resulting image. This sequence links labeling to interpretable visual data.
In cancer research, Wide-field Fluorescence Microscopy can address several complementary questions: how tumor cells are shaped, whether biomarkers are expressed, how cells proliferate, and how cells interact within tissue. Because the approach supports both cellular and tissue-level observations, it helps organize visual comparisons across samples or experimental conditions.
When evaluating an experimental treatment, researchers can use the images to document cellular responses and compare specimens under different conditions. The observations may include tumor-cell morphology, marker-associated fluorescence, proliferation-related labeling, or cellular relationships in tissue. Wide-field Fluorescence Microscopy therefore contributes visual evidence for treatment-response studies and comparative cancer analyses.