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Biology
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
JoVE Journal
Biology
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JoVE Journal Biology
Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Full Text
5,555 Views
15:00 min
November 30, 2006

DOI: 10.3791/120-v

Meg Bentley1, Randy King1

1Dept. of Cell Biology,Harvard Medical School

Actually what I'm gonna do, mount it in here first and then swap out You. So now I'm gonna aspirate try and be as gentle as possible.Okay. A few seconds just holding.Okay.Alright.

So the important features of our lifestyle imaging grid are that, so it's an inverted scope, which means the objective, the samples here in the objective is underneath. So the objective is pointing up and the whole scope, or practically the whole, practically the whole scope, including the objectives and the sample are at 37 degrees. So everything in this plastic box is at 37 degrees.

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