Laboratory Procedure
Laboratory Procedure - Related Videos
06:03
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
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Cited by 11 •
2016
Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.
09:12
Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats
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Cited by 3 •
2019
We present three protocols that assess different forms of impulsivity in rats and other small mammals. Intertemporal choice procedures evaluate the tendency to discount the value of delayed outcomes. Differential reinforcement of low rates and feature-negative discrimination evaluate response inhibition capacity with and without punishment for inappropriate responses, respectively.
09:54
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures
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Cited by 21 •
2016
Although researchers are generally knowledgeable about procedures and safety precautions required for biosafety level 1 or 2 (BSL-1/2) experiments, they may not be familiar with experimental procedures in BSL-4 suit laboratories. This article provides a detailed visual demonstration of BSL-4 suit laboratory systems check, laboratory entry, movement, and exit procedures.
09:36
Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 4. Medical Imaging Procedures
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Cited by 8 •
2016
Here, we present an overview of the preparation and animal handling procedures required to safely perform medical imaging in an animal biosafety level 4 laboratory. Computed tomography of a mock-infected guinea pig illustrates these procedures that may be used to evaluate the disease caused by a high consequence pathogen.
05:21
Training Laboratory Rabbits to Refine Routine Husbandry Procedures
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Cited by 2 •
2024
Training protocols involving positive reinforcement for routine husbandry procedures in rabbits were developed and proved to be successful, as the rabbits reliably showed the trained behaviors at the weekly general examination. Picking the rabbits up with the transport box was less aversive than the conventional handling technique.
FAQs
Controlled conditions make important variables, such as temperature and reaction time, consistent from one trial to the next. This consistency reduces unexplained variation and helps researchers determine whether differences in results arise from the chemistry being studied rather than from changes in technique. It also allows findings to be compared more confidently across experiments or laboratories.
The sequence of operations can determine whether materials are prepared correctly, equipment is used at the appropriate stage, and observations are recorded at meaningful points. Following the intended order limits avoidable experimental error and makes unexpected outcomes easier to trace. If a result is unusual, the documented sequence provides a structured basis for identifying where the process may have changed.
Safety is supported by specifying how materials are prepared, handled, and processed under defined conditions. A clear sequence reduces improvisation, which can otherwise increase exposure to hazards or lead to unsuitable equipment use. Procedures also communicate expected operations to students and researchers, helping them carry out chemical work consistently while maintaining attention to potential risks.
The required operations depend on the chemical objective. Synthesis procedures organize steps for producing a substance, whereas separation procedures emphasize isolating components. Identification procedures focus on observations or measurements that distinguish materials, and quantitative analysis requires measurements suitable for determining amounts. In each case, the procedure aligns preparation, equipment use, controlled conditions, and records with the intended outcome.
It should identify how materials are prepared, which equipment is used, and how conditions such as temperature and reaction time are controlled. The sequence of operations should be clear enough that each step can be followed consistently. The procedure should also indicate when observations or measurements are recorded, creating a complete basis for evaluating the experiment or analysis afterward.
They are most useful when an experiment or analysis produces an outcome that differs from expectations. Researchers can compare the recorded work with the prescribed sequence, material preparation, equipment use, controlled conditions, and observations. This comparison helps separate procedural changes from possible chemical behavior and supports a more systematic response than simply repeating the work without reviewing what occurred.
Documentation gives separate laboratories a shared account of the operations, conditions, measurements, and observations used in the work. When those details are sufficiently clear, researchers can reproduce the process and compare outcomes rather than relying on informal descriptions. This supports consistent teaching, collaborative research, and evaluation of whether differences between results reflect the chemistry or variations in execution.