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Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
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Genetics
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JoVE Journal Genetics
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

DOI:
10.3791/54446-v

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06:03 min

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September 20, 2016

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, ,
1Super-Bacteria Research Center,Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Microbiomics and Immunity Research Center,Korea Research Institute of Bioscience and Bioengineering (KRIBB), 3Department of Biotechnology,The Catholic University of Korea

Chapters

  • 00:05Title
  • 00:36Initial Cultivation and Stress Adaptation
  • 02:58Single-colony Isolation of the Stress-adapted Strain
  • 04:02Results: High Succinate Stress Adaptation
  • 05:09Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.

Tags

Keywords: Adaptive Laboratory EvolutionMicroorganismChemostatE. ColiAerationAgitationOptical DensityHigh Stress MediumStressorGlycerolAgar Plate

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