M/z 31
M/z 31 - Related Videos
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
0 Views •
Cited by 36 •
2010
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
01:16
Other Nuclides: 31P, 19F, 15N NMR
0 Views •
2024
Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research. While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a high...
05:57
Quantitative 31P NMR Analysis of Lignins and Tannins
0 Views •
Cited by 45 •
2021
31P NMR is a powerful tool for the structural elucidation of polyphenols. This fast, easy, precise, quantitative, and highly reproducible analytical procedure, that allows for the quantification and differentiation of the different types of hydroxy, phenolic, and carboxylic groups in lignins and tannins has now become a routine analytical tool.
![Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-<sup>13</sup>C]Pyruvate and <sup>13</sup>C/<sup>31</sup>P NMR Spectroscopy](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63188.jpg%3Fv%3D2026-09-01%252019%253A57%253A02&w=750&q=75)
14:56
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
0 Views •
2023
We describe an experimental setup for administrating hyperpolarized 13C-labeled metabolites in continuous perfusion mode to an isolated perfused mouse heart. A dedicated 13C-NMR acquisition approach enabled the quantification of metabolic enzyme activity in real-time, and a multiparametric 31P-NMR analysis enabled the determination of the tissue ATP content and pH.
09:40
Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
0 Views •
Cited by 9 •
2017
This work demonstrates the feasibility of an in vivo phosphorus-31 magnetic resonance spectroscopy (31PMRS) technique to quantify mitochondrial oxidative phosphorylation (OXPHOS) capacity in human skeletal muscle.
FAQs
The same mass-to-charge value does not identify a single ion composition. In oxygen-containing compounds, ions such as CH3O+ and CH2OH+ can both occur near m/z 31 after electron-ionization fragmentation. Their shared signal must therefore be interpreted with the compound’s other fragment peaks, molecular-ion peak, and overall reference spectrum rather than assigned to one structure automatically.
Electron ionization can break an oxygen-containing molecule into charged fragments, including species associated with cleavage near an oxygen-containing functional group. For suitable alcohols, ethers, and related compounds, this process may generate ions near m/z 31. The resulting peak reflects both the available molecular structure and the fragmentation pathways favored under the applied ionization conditions.
Peak intensity depends on molecular structure, the relative ease of competing fragmentation pathways, and instrument conditions. Consequently, a strong or weak m/z 31 signal has significance only within the complete spectrum. Comparing its intensity with accompanying ions and the molecular-ion peak helps determine whether the signal supports an oxygen-containing structural interpretation.
The molecular-ion peak provides evidence related to the intact ion, whereas m/z 31 reflects a smaller charged fragment formed during ionization. Examining both connects a proposed fragment to the parent molecule and reduces overinterpretation of an isolated signal. Agreement between the fragment pattern, molecular-ion information, and a reference spectrum strengthens the structural assignment.
First, note the m/z 31 peak and its relative intensity. Next, examine accompanying fragment ions and determine whether a molecular-ion peak is present. Consider possible oxygen-containing compositions, including CH3O+ or CH2OH+, then compare the complete pattern with reference spectra. This workflow treats the signal as supporting evidence and keeps the assignment tied to the full spectrum.
Chemists can use the signal when evaluating alcohols, ethers, and other compounds containing relevant oxygen-based structural features. It helps indicate that particular fragmentation behavior may be present, especially under electron-ionization conditions. Because several structures can produce similar ions and intensities vary, the peak is most useful for narrowing interpretations rather than assigning a compound by itself.