Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

The overall goal of this In Vivo magnetic resonance technique and exercise protocol is to establish a robust and standardized method for the noninvasive measurement of mitochondrial oxidative phosphorylation capacity. Skeletal muscle mitochondrial oxidative phosphorylation capacity can be measured noninvasively with In Vivo phosphorous 31 magnetic resonance spectroscopy. This standardized technique will facilitate the development of targeted therapies to improve oxidative phosphorylation capacity.

The main advantages of this technique are that it is noninvasive allowing for serial investigation, and that it is well tolerated by individuals spanning a wide range of functional capabilities. Generally, individuals new to this method will struggle because the equipment set up and subject positioning are technically intensive and crucially important to obtain good results. Prior to imaging, first obtain written and informed consent from the subject and thoroughly screen them for MRI safety.