In Vitro Cell Culture
In Vitro Cell Culture - Related Videos
06:31
In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
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Cited by 13 •
2016
The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.
05:44
In vitro Cell Culture Model for Toxic Inhaled Chemical Testing
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Cited by 25 •
2014
This protocol is designed to demonstrate exposure method of cell cultures to inhaled toxic chemicals. Exposure of differentiated air-liquid interface (ALI) cultures of airway epithelial cells provides a unique model of airway exposure to toxic gases such as chlorine. In this manuscript we describe effect of chlorine exposure on air-liquid interface cultures of epithelial cells and submerged culture of cardiomyocytes. In vitro exposure systems allow important mechanistic studies to evaluate...
09:11
Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
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Cited by 35 •
2016
Zika Virus (ZIKV), an emerging pathogen, is linked to fetal developmental abnormalities and microcephaly. The establishment of an effective infectious cell culture system is crucial for studies of ZIKV replication as well as vaccine and drug development. In this study, various virological assays pertaining to ZIKV are illustrated and discussed.
09:48
In Vitro Resident Memory CD8 T Cell Differentiation Using Epithelial Organoid-T Cell Co-culture System
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2026
To study CD8 T cell differentiation and function in vitro, CD8 T cells can be isolated from the mouse to be co-cultured long-term with preformed infected murine vaginal epithelial organoids. Here, we describe this process and assess the acquisition of resident memory T cell markers upon co-culture.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
FAQs
Nutrient-containing media, temperature, gases, pH, and sterility create the conditions that determine whether cells remain viable and display interpretable behavior. Because these factors can be controlled outside the organism, researchers can examine cellular responses under defined conditions and relate changes in division, differentiation, communication, or organization to specific experimental variables.
Cultured cells and tissues provide accessible systems for examining how signaling pathways relate to lineage specification, the process by which cells acquire distinct developmental identities. Researchers can monitor how cells respond as they divide, differentiate, communicate, or organize, helping connect altered signals or conditions with changes in developmental outcomes.
In vitro models make cells and tissues more accessible while allowing researchers to study responses under defined laboratory conditions. This controlled setting can clarify developmental mechanisms involving signaling, lineage specification, and morphogenesis. However, the models are used to examine cellular and tissue behaviors directly, rather than replacing the broader developmental context provided by an organism.
Cells are placed in nutrient-containing culture media and maintained in a laboratory environment with appropriate temperature, gases, pH, and sterility. Researchers then monitor the cultures as cells divide, differentiate, communicate, or organize. This workflow supports controlled observation of cellular responses and provides a basis for testing how genetic or environmental changes affect development.
Observable outcomes include changes in cell division, differentiation, communication, and organization. In developmental biology, these responses can indicate how signaling pathways and lineage specification contribute to morphogenesis, the formation of tissue or body structure. Monitoring such outcomes helps researchers connect experimental conditions with developmental mechanisms and compare responses across defined culture settings.
Researchers use cultured cells and tissues to investigate signaling pathways, lineage specification, morphogenesis, and the effects of genetic or environmental changes. These models make developmental processes more accessible for controlled study while supporting disease modeling, drug testing, and tissue engineering. Their value lies in linking cellular responses to broader developmental and applied research questions.