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JoVE Journal
Bioengineering

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An In Vitro Organ Culture Model of the Murine Intervertebral Disc
 

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Article doi: 10.3791/55437
April 11th, 2017

Summary April 11th, 2017

Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.

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