Embryo Grafting
Embryo Grafting - Related Videos
11:48
Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
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Cited by 2 •
2016
By grafting beads soaked in growth factors or specific inhibitors of signaling pathways into developing embryos it is possible to directly test their effects in vivo. In this protocol beads are grafted into the limb bud to determine the effects of these molecules on gene expression and signal transduction.
09:57
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
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Cited by 11 •
2015
Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.
11:14
Assay for Neural Induction in the Chick Embryo
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Cited by 1 •
2009
Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
03:33
Visualization of Neural and Vascular Networks in a Chicken Embryo
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2025
Source: Delalande, J., et.al. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (2015)This video demonstrates the transplantation of a GFP-labeled donor neural tube from a stage-matched transgenic chicken embryo into a recipient embryo at the level of somites one to seven, followed by vascular labeling using a lipophilic fluorescent dye. The combined approach allows for direct...
FAQs
The compatible rootstock provides water, minerals, and growth support while the graft becomes established. This support is especially important because the isolated embryo no longer develops within an intact seed. Successful contact allows development to continue under controlled conditions, making the rootstock a functional source of support rather than merely a physical attachment surface.
Compatibility determines whether the transferred embryo can establish contact with rootstock tissue and continue growing. An unsuitable rootstock may fail to provide effective support, whereas a compatible one can sustain development after excision. Studying these outcomes also helps researchers examine the biological basis of graft establishment and the relationship between embryo development and supporting tissues.
Wide crosses can produce hybrid embryos that abort before the seed matures. Embryo grafting allows such immature embryos to be removed from the failing seed context and supported on compatible rootstock tissue. In genetics, this can recover hybrids that might otherwise be lost, enabling researchers to retain and examine combinations of traits from different plants.
Differences in whether excised embryos establish and continue developing can provide information about early growth requirements and genetic control. The method separates embryo performance from the normal seed environment, allowing researchers to examine development, compatibility, and the maintenance of valuable genotypes. These observations can clarify why some embryos mature normally while others become nonviable.
The procedure begins under sterile conditions by carefully excising the immature embryo. Researchers then place it in contact with compatible rootstock tissue and maintain conditions that allow the graft to establish. The embryo is monitored for continued development, while the rootstock supplies water, minerals, and growth support. Careful handling is essential because the embryo is removed before normal seed maturation.
Researchers apply the technique when embryos are threatened by abortion, fail to mature after a wide cross, or remain constrained by seed dormancy. It can also help maintain valuable genotypes that cannot develop normally inside intact seeds. The resulting plants or developmental observations support studies of inheritance, hybrid recovery, embryo development, and compatibility.