Cryopreserved Monocytes
Cryopreserved Monocytes - Related Videos
06:46
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
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Cited by 58 •
2016
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
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Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
08:31
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Cited by 19 •
2016
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
11:10
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
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Cited by 23 •
2011
Preimplantation embryos may be cryopreserved after placement into a hypertonic cryoprotective solution to cause cellular dehydration. After equilibration, ice crystal formation is induced in the solution surrounding the embryo. Further dehydration occurs as the embryo is slowly cooled to subzero temperatures before plunging into liquid nitrogen for storage.
FAQs
Dimethyl sulfoxide, commonly abbreviated DMSO, serves as a cryoprotectant during preparation for freezing. The cells are suspended in this protective medium before controlled cooling, which helps preserve monocytes during storage at very low temperatures. Its role is therefore linked to maintaining cells for later recovery and experimentation rather than directly activating or differentiating them.
Controlled cooling and rapid thawing address different stages of cryogenic stress. Gradual, regulated cooling helps limit freezing-related injury before storage, while careful rapid thawing reduces damage associated with ice during recovery. Together, these conditions support the return of monocytes to a state suitable for direct assessment or subsequent culture-based experiments.
After thawing, monocytes may be examined directly or cultured with defined signals that promote differentiation into macrophages or dendritic cells. This choice changes the cellular model available for study: direct assessment focuses on recovered monocytes, whereas differentiation provides specialized cell types for investigating immune activation, pathogen responses, or inflammatory behavior.
Cryopreserved monocytes can make experiments more standardized by reducing dependence on same-day fresh blood samples. Stored cells allow investigators to plan studies around a prepared, accessible source of blood-derived innate immune cells. This consistency is especially useful when comparing inflammatory or infection-related responses across experiments or evaluating therapeutic effects under more uniform conditions.
A typical workflow begins by suspending monocytes with DMSO, cooling them under controlled conditions, and maintaining them in liquid nitrogen. For an experiment, the stored cells are thawed carefully and rapidly, then either assessed directly or placed in culture with defined differentiation signals. The selected downstream route depends on the immune response or cell type under investigation.
The key preservation elements are a DMSO-containing suspension, controlled cooling, liquid-nitrogen storage, and careful rapid thawing. Each contributes to maintaining cells through preparation, storage, and recovery. The source material does not require a single downstream assay, because thawed monocytes may be evaluated directly or directed toward macrophage or dendritic-cell cultures.
These cells support studies of pathogen recognition, cytokine production, antimicrobial responses, and broader immune activation. In infection research, investigators can use them to examine how innate immune cells respond to pathogen-related stimulation. Their availability after storage also supports comparisons among experimental conditions without relying exclusively on freshly collected blood for every study.
Defined culture signals can convert thawed monocytes into macrophages or dendritic cells, extending experiments beyond the starting cell population. This enables investigation of immune behavior in models representing distinct differentiated states, including responses relevant to inflammation, pathogen recognition, antimicrobial activity, and therapeutic testing. The resulting system helps connect cell preparation with specific host-response questions.