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Biology

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Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
 

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Article DOI: 10.3791/3713
December 12th, 2011

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Summary December 12th, 2011

Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.

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