Murine Macrophages
Murine Macrophages - Related Videos
01:36
Wnt5A-Mediated Enhancement of Bacterial Clearance in Murine Macrophages
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2026
Take an adherent culture of murine macrophages.Treat the test well with a buffer containing Wnt5A, a signaling molecule, and the control with buffer only.Infect with a bacterial pathogen and incubate.In the test cells, Wnt5A activates a signaling cascade that enhances cytoskeletal reorganization to boost bacterial uptake into phagosomes, whereas control cells lacking this signaling internalize fewer bacteria.Wash to remove non-internalized bacteria, then incubate with a medium containing...
07:05
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
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Cited by 2 •
2017
Target-specific probes represent an innovative tool for analyzing molecular mechanisms, such as protein expression in various types of disease (e.g., inflammation, infection, and tumorigenesis). In this study, we describe a quantitative three-dimensional tomographic assessment of intestinal macrophage infiltration in a murine model of colitis using F4/80-specific fluorescence-mediated tomography.
03:36
RNA Extraction from Listeria monocytogenes Harvested from Infected Murine Macrophages
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2026
Source: Sigal, N., et al. RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells. J. Vis. Exp. (2016)This video demonstrates RNA extraction from Listeria monocytogenes isolated from infected murine macrophages. The protocol preserves RNA, lyses cells using phenol–chloroform and heat, and separates RNA by centrifugation. Salt and ethanol, combined with cold incubation, promote RNA precipitation. The resulting RNA captures host-induced gene expression, revealing...
05:05
In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
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2025
This video demonstrates the activation of a mouse's peritoneal macrophages using intravenous immunoglobulin and bacterial lipopolysaccharide. The co-stimulation activates the peritoneal macrophages into anti-inflammatory macrophages possessing immune regulatory activity.
08:21
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
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Cited by 78 •
2015
We describe here a simple protocol to isolate murine peritoneal macrophages. This procedure is followed by RNA extraction to carry out gene expression analysis upon Toll-like receptors stimulation.
FAQs
Pattern-recognition receptors detect microbial patterns and initiate signaling that coordinates uptake and downstream immune responses. During phagocytosis, cells internalize pathogens or particulate material, while released cytokines and other mediators influence inflammation and tissue repair. Studying these linked events helps distinguish pathogen capture from the broader inflammatory program triggered after recognition.
Cytokine release provides an indicator of how macrophages respond after encountering pathogens or microbial products. These mediators help shape antimicrobial responses and inflammation, while also contributing to tissue-repair processes. Measuring activation alongside cytokine production therefore connects cellular recognition and uptake with the wider immune effects that may influence infection outcomes.
Bone marrow-derived cells, peritoneal macrophages, and established cell lines provide different experimental systems for examining host defense. Their use allows investigators to expose macrophages to defined bacteria, viruses, parasites, or microbial products under controlled conditions. Selecting among these sources helps align the model with questions about uptake, signaling, activation, or host susceptibility.
A typical study begins with macrophages obtained from bone marrow, peritoneal cavities, or an established cell line. Investigators then expose the cells to a pathogen or microbial product under controlled conditions and examine outcomes such as pathogen uptake, inflammatory signaling, macrophage activation, or susceptibility. This workflow links a defined challenge to measurable host responses.
The system can be used with bacteria, viruses, parasites, or microbial products, depending on the research question. Controlled exposure lets investigators examine how macrophages respond to distinct infectious or microbial stimuli while focusing on uptake, signaling, and activation. These comparisons support analysis of how different challenges shape innate immune responses.
These models help investigate how pathogens are taken up, how inflammatory signaling is initiated, and how macrophage activation affects host susceptibility. They also support evaluation of immune-modulating treatments and can clarify mechanisms that may later be examined in more complex mammalian models. Consequently, they connect cellular innate immunity with broader infection research.