Scintillation Vial
Scintillation Vial - Related Videos
10:52
Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
0 Views •
Cited by 21 •
2016
Phosphoinositides are signaling lipids whose relative abundance rapidly changes in response to various stimuli. This article describes a method to measure the abundance of phosphoinositides by metabolically labeling cells with 3H-myo-inositol, followed by extraction and deacylation. Extracted glycero-inositides are then separated by high-performance liquid chromatography and quantified by flow scintillation.
04:25
Substrate Oxidation Assay in Cultured Cells: An In Vitro Assay to Quantify Substrate Oxidation in Cells by Measuring Radioactive Signals from Trapped CO2
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2025
This video demonstrates a quantification technique for CO2 released during substrate oxidation using 14C-radiolabeled substrates. The released 14CO2 is trapped in an alkaline solution and quantified by a scintillation counter. The oxidation of different substrates varies between tissues and reflects the pathophysiological condition of the tissue.
02:54
DNA Synthesis Assay: A Technique to Assess DNA Synthesis in Proliferating Cells Using Radioactive Tritiated Thymidine Incorporation
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2023
In this video, we determine the proliferative capacity of neuronal progenitor cells by measuring the incorporation of radioactive tracer tritiated thymidine into the DNA of cells. This technique can be used to study cell proliferation after treatment with certain drugs and growth factors.
10:01
Measurement of Carbon Dioxide Production from Radiolabeled Substrates in Drosophila melanogaster
0 Views •
Cited by 7 •
2016
This paper describes a method for the measurement of fuel oxidation in Drosophila melanogaster in which trace amounts of specific radiolabeled metabolic substrates are fed to flies. The exhaled radiolabeled CO2 that is a produced from fuel oxidation is collected and measured.
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
0 Views •
2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
FAQs
Radiation emitted by the sample transfers energy to molecules in the scintillation fluid, causing brief flashes of light. Photomultiplier detectors capture and convert those flashes into measurable signals. This energy-transfer pathway allows liquid scintillation counting to detect and quantify radioactive compounds, including low-energy beta-emitting materials used in biomedical and laboratory medicine studies.
Vial material and transparency can influence how effectively emitted light reaches the photomultiplier detectors. The container must also remain chemically compatible with the scintillation fluid and sample. Choosing an appropriate vial therefore supports reliable light transmission, protects the contents during measurement, and helps maintain counting efficiency across repeated laboratory assays.
Secure sealing helps contain the radioactive sample and scintillation fluid during storage, transport, and measurement. Because the vial holds both the specimen and the counting cocktail, a suitable closure supports controlled sample handling while reducing the risk of leakage or loss of contents. This is particularly relevant when laboratory workflows involve multiple radiotracer samples.
A laboratory workflow places the radioactive sample and scintillation fluid in a compatible, light-transmitting vial, then secures the container before measurement. The prepared vial is positioned for detection, where radiation-driven light flashes are converted into signals by photomultiplier detectors. Consistent preparation helps preserve sample integrity and supports comparable measurements between specimens.
Scintillation vials support radiotracer studies, pharmacokinetics, metabolic research, and clinical assay development. In these applications, the measured signal provides information about radioactive compounds in a sample, helping investigators follow tracer behavior, examine metabolism, or evaluate assay performance. Their use connects radiation measurement with laboratory medicine and broader biomedical research.
Low-energy beta-emitting compounds can be detected and quantified through the light produced when sample radiation transfers energy to the scintillation fluid. The vial provides the compatible, transparent setting needed for this measurement process, while the detector records the resulting flashes. This makes the approach useful when biomedical experiments depend on measuring small radioactive signals.