Intrinsic Optical Signals
Intrinsic Optical Signals - Related Videos
11:24
Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging
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Cited by 19 •
2012
A method is described for labeling neurons with fluorescent dyes in predetermined functional micro-domains of the neocortex. First, intrinsic signal optical imaging is used to obtain a functional map. Then two-photon microscopy is used to label and image neurons within a micro-domain of the map.
08:30
Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
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Cited by 9 •
2011
This article presents the protocols of intrinsic optical signals and flavoproteins autofluorescence signals imaging to map odor-evoked activities at the surface of the olfactory bulb in mice.
09:43
Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
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Cited by 10 •
2017
We demonstrate the transmission of multiple independent signals through a multimode fiber using wavefront shaping employing a single spatial light modulator. By modulating the wavefront for each signal individually, spatially separated foci are transmitted. Potential applications are multiplexed data transfer in communications engineering and endoscopic light delivery in biophotonics.
02:18
Intrinsically Disordered Proteins
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2020
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
FAQs
Neural activation changes metabolism, blood flow, blood volume, and oxygenation within the tissue. These physiological shifts modify how cortical tissue absorbs and scatters light, so the detected reflected-light pattern changes even without adding a fluorescent label. Because several processes contribute to the optical response, measurements can reveal activity-related vascular and metabolic consequences rather than neuronal firing alone.
Neurovascular coupling links neural activity with local changes in blood flow, blood volume, and oxygenation. Intrinsic optical measurements therefore provide information about how vascular responses accompany functional activity in the cortex. This relationship is scientifically useful for studying normal cortical organization, but it also means that the optical signal reflects interacting neural and vascular processes rather than an isolated electrical event.
Intrinsic optical imaging monitors activity-related changes in tissue light properties without requiring an added label, whereas electrophysiological recordings directly complement it by measuring neural electrical activity. Fluorescence-based techniques provide another complementary approach that relies on fluorescent signals. Using these methods together can help distinguish spatial patterns of tissue optical change from electrical or fluorescence-defined measures of neural function.
Measurements use cameras or specialized microscopes to detect reflected light from neural tissue. The tissue must be exposed or otherwise optically accessible so activity-related changes in absorption and scattering can reach the imaging system. This setup supports mapping across the observed region and is especially valuable when investigators need high spatial resolution without introducing a fluorescent marker.
The technique can map sensory representations and examine cortical organization by showing where activity-related optical changes occur across accessible tissue. It also supports investigations of neurovascular coupling, including the relationship between neural function and accompanying changes in blood flow, blood volume, and oxygenation. These applications make it useful for studying both spatial patterns and physiological responses in the brain.
Disease-related changes can be examined through alterations in the optical responses associated with neural activity, metabolism, blood flow, blood volume, or oxygenation. By mapping these signals across optically accessible neural tissue, investigators can compare functional organization and neurovascular responses under different conditions. Electrophysiological or fluorescence-based measurements can complement the imaging results and provide additional context for interpretation.