Pressure Myography
Pressure Myography - Related Videos
07:25
Assessing Murine Resistance Artery Function Using Pressure Myography
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Cited by 18 •
2013
In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
11:02
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
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Cited by 8 •
2012
Measurements of Kv7 (KCNQ) potassium channel activity in isolated arterial myocytes (using patch clamp electrophysiological techniques) in parallel with measurements of constrictor/dilator responses (using pressure myography) can reveal important information about the roles of Kv7 channels in vascular smooth muscle physiology and pharmacology.
10:48
Assessing Myogenic Response and Vasoactivity In Resistance Mesenteric Arteries Using Pressure Myography
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Cited by 24 •
2015
Pressure myography is used to assess vasoactivity of small arteries that develop sustained constriction when pressurized. This manuscript provides a detailed protocol to assess in isolated segments of small mesenteric arteries from rats, vasoactivity and the effect of intraluminal pressure on vascular diameter.
07:26
Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Cited by 5 •
2013
The laser heated diamond anvil cell combined with synchrotron micro-diffraction techniques allows researchers to explore the nature and properties of new phases of matter at extreme pressure and temperature (PT) conditions. Heterogeneous samples can be characterized in situ under high pressure by 2D mapping and combined powder, single-crystal and multigrain diffraction approaches.
02:34
Vapor Pressure
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2020
When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase molecules move randomly about, they will occasionally collide with the surface of the condensed phase, and in some cases, these collisions will result in the molecules re-entering the condensed phase. The change from the gas phase to the liquid is called condensation. When the rate of condensation becomes equal to the rate of vaporization, neither the amount of the liquid nor the amount of the vapor...
FAQs
In pressure myography, raising intraluminal pressure stretches the vessel wall and activates pressure-sensitive signaling in the smooth muscle. This response can alter intracellular calcium, which then influences contraction or relaxation and changes vessel diameter. Comparing diameter across controlled pressures therefore reveals how an isolated vessel converts mechanical stimulation into vascular tone.
Intracellular calcium provides a functional link between pressure-sensitive signaling and smooth muscle behavior. When pressure-related signaling changes calcium within the vessel wall, the muscle may contract or relax, producing a measurable change in diameter. Tracking this diameter response helps researchers examine how cerebral vessels regulate tone under defined mechanical conditions.
The preparation applies intraluminal pressure while excluding flow, allowing the vessel response to be examined under a controlled pressure condition. This focuses the measurement on how wall stretch and pressure-sensitive signaling affect smooth muscle tone. The resulting diameter changes provide a direct readout of pressure-related vascular behavior in the isolated vessel.
Diameter measurements are interpreted together with the pressure condition that produced them. A pressure-associated decrease in diameter indicates increased smooth muscle contraction, whereas widening indicates relaxation under the tested condition. Repeating measurements across controlled pressures helps characterize tone as a dynamic response rather than treating vessel size as a fixed structural property.
A typical experiment begins with an isolated small vessel, followed by cannulation and application of controlled intraluminal pressure. The vessel remains pressurized without flow while its diameter is observed as smooth muscle responds. Measurements collected across the selected pressure conditions can then be used to evaluate contraction, relaxation, and vascular tone.
Cerebral arteries and arterioles provide a way to examine vascular behavior relevant to brain perfusion. With pressure myography, researchers can assess myogenic regulation and vascular reactivity directly in these vessels. The findings help connect smooth muscle responses and diameter changes with mechanisms that influence cerebral blood flow and neurovascular function.
In neuroscience research, the technique can be used to investigate how cerebral vessels respond to controlled pressure and how those responses shape vascular tone. Such measurements support studies of myogenic regulation, vascular reactivity, and cerebral blood flow. They also provide experimental context for understanding vascular mechanisms involved in disorders affecting brain perfusion.