Probe Holder
Probe Holder - Related Videos
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
02:22
Positioning Laser Doppler Probe: A Technique to Implant Probe Over Middle Cerebral Artery for Blood Flow Measurement in Murine Model
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2025
This video describes the surgical procedure to immobilize the laser Doppler probe over the murine brain's middle cerebral artery, or MCA. The probe helps to measure the blood flow in the brain's microvasculature by measuring the Doppler shift caused due to the motion of red blood cells within the artery.
08:07
The Microfluidic Probe: Operation and Use for Localized Surface Processing
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Cited by 5 •
2009
In this video we present the microfluidic probe1 (MFP). We explain in detail how to assemble the MFP, mount it atop an inverted microscope, and align it relative to the substrate surface, and finally show how to use it to process a substrate surface immersed in a buffer solution.
05:57
Biomembrane Force Probe to Quantitate Receptor-Ligand Interactions
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2025
In this video, biomembrane force probes (BFPs) are used to measure the forces between receptors and ligands. This technique can detect interactions by monitoring the pressure needed to break the bonds between the pMHC conjugated on the probe beads and the TCRs on the target cells.
07:55
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
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Cited by 4 •
2019
A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.
FAQs
Stability comes from maintaining a fixed mechanical relationship between the probe and its target while limiting vibration and unintended displacement. This steadiness helps preserve the probe’s angle and depth during recording, stimulation, microinjection, or imaging. As a result, measured signals or delivered interventions are more consistent, making differences between experimental preparations easier to interpret.
These components connect the probe to a stereotaxic frame or micromanipulator and provide different forms of positional control. Clamps grip the probe, adapters accommodate interfaces, and adjustable mounts help set its angle, depth, or movement. Choosing the appropriate combination allows researchers to match the holder to the instrument and the spatial demands of the experiment.
Angle, depth, movement, and resistance to vibration are central alignment factors. If the holder does not maintain these conditions, the probe may shift relative to the target, reducing targeting accuracy or signal consistency. Careful alignment therefore affects both experimental reliability and tissue protection, especially when the probe must remain precisely positioned throughout a measurement or intervention.
The suitable holder depends on the probe’s function and the positional control that function requires. Recording and imaging benefit from stable placement that supports consistent signals or observations, while stimulation and microinjection require controlled positioning relative to the target. In each case, the holder must support the needed angle, depth, and movement without damaging the probe or tissue.
A typical workflow is to secure the probe with a clamp or compatible adapter, attach the holder to the stereotaxic frame or micromanipulator, and align the assembly with the intended target. The researcher then controls the probe’s angle, depth, and movement while checking that the placement remains stable. This sequence supports reproducible positioning across preparations.
A specialized holder is particularly valuable when an experiment depends on accurate targeting, repeated positioning, or stable measurements from a delicate probe. It can support electrophysiological recording, neural stimulation, microinjection, or imaging when ordinary support would allow vibration or displacement. Selecting and aligning the holder carefully can improve reproducibility while reducing risks to both probe integrity and tissue.