Thrombus Visualization
Thrombus Visualization - Related Videos
02:05
Visualization of a Cerebral Thrombus in a Mouse Using Micro-Computed Tomography Imaging
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2025
Source: Kim, D., et. al. Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli. J. Vis. Exp. (2016)This video demonstrates visualization of cerebral thrombus in mice using micro-computed tomography (micro-CT) imaging. A cerebral thrombus mouse model is injected with fibrin-targeted gold nanoparticles acting as a contrasting agent. The mouse is placed on the bed of a micro-CT machine, and images are acquired from multiple angles.
09:01
Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
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Cited by 4 •
2017
The ear model of the hairless SKH1-Hrhr mouse enables intravital fluorescence microscopy of microcirculation and phototoxic thrombus induction without prior surgical preparation in the examined microvascular bed. Therefore, the ear of the hairless mouse is an excellent in vivo model to study the complex interactions during microvascular thrombus formation, thrombus evolution, and thrombolysis.
05:54
Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation
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2025
This video demonstrates the intravital fluorescence microscopy technique to study thrombus formation in a mouse model. The mouse is injected with a fluorophore-labeled polysaccharide to monitor blood circulation. Upon illumination with a high-intensity light, the phototoxic induction of an injury in the microvasculature of the ear leads to the formation of a thrombus, which is confirmed by visualizing the obstruction in the blood flow.
08:50
Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
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2026
This work describes a microfluidic assay that uses shear stress to trigger thrombus formation in blood and characterizes the thrombus by multiple dimensions of readout. The assay can measure the prothrombotic tendency of blood samples, and is thus useful in disease diagnosis, drug discovery, as well as basic mechanistic studies related to thrombosis.
11:18
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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Cited by 29 •
2013
Here we report an experimental technique of fluorescence intravital microscopy to visualize heterotypic platelet-neutrophil interactions on the activated endothelium during vascular inflammation and thrombus formation in live mice. This microscopic technology will be valuable to study the molecular mechanism of vascular disease and to test pharmacologic agents under pathophysiological conditions.
FAQs
Labeled platelets and fibrin provide complementary signals during thrombus visualization. Platelet labeling indicates where cellular components accumulate, while fibrin labeling helps identify the protein network that stabilizes the clot. Tracking both signals over time can distinguish changes in thrombus composition and organization, clarifying how a clot develops at a damaged cerebral vessel.
Blood-flow measurements place clot growth in its vascular context. Imaging can show how platelet and fibrin accumulation coincides with changes in circulation, including obstruction within a cerebral vessel. This relationship helps researchers connect thrombus development with reduced oxygen delivery and assess whether an intervention changes the clot or restores movement of blood.
Serial imaging can show whether a thrombus continues to grow, remains obstructive, or begins to clear after treatment. Comparing clot signals, vessel obstruction, and blood flow before and after antithrombotic or thrombolytic intervention provides a dynamic measure of response. In neuroscience studies, these observations can be linked to changes in brain injury and recovery.
A workflow generally combines imaging with analysis of labeled platelets, fibrin, and blood flow at a site of vessel damage. Researchers follow these signals as they accumulate and change, then relate the observations to vessel blockage and downstream effects. The resulting time-resolved view supports analysis of formation, structure, clearance, and treatment response.
This approach is particularly useful for studying ischemic stroke, cerebrovascular disorders, and therapies intended to preserve brain function. It allows investigators to examine clot behavior within cerebral vessels rather than considering neuronal injury separately. Linking vascular events with oxygen-delivery disruption and subsequent recovery can help characterize how thrombi contribute to brain damage.
Researchers can interpret where thrombi form, how their platelet and fibrin signals change, whether circulation becomes obstructed, and whether the clot clears. These measurements provide evidence about the relationship between vascular blockage and neuronal damage. They also support comparisons of antithrombotic or thrombolytic responses, helping evaluate mechanisms and potential strategies for limiting brain injury.