What are fibrin-targeted gold nanoparticles?

They are nanoparticles designed to bind specifically to fibrin, enhancing imaging of thrombi.

How is the imaging process initiated?

The imaging process begins five minutes after the injection of the nanoparticles.

What is the role of micro-computed tomography in this study?

Micro-CT is used to obtain high-resolution images of the thrombus.

Why is sonication important in this protocol?

Sonication ensures a homogeneous suspension of the nanoparticles for effective imaging.

What is the significance of thrombus imaging?

Thrombus imaging is essential for understanding and diagnosing vascular diseases.

What experimental model is used in this study?

The study uses experimental animals for the injection and imaging process.

Visualization of a Cerebral Thrombus in a Mouse Using Micro-Computed Tomography Imaging

JoVE Encyclopedia of Experiments

Neuroscience

0 views • 2:05 min • June 17th, 2025

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To image the thrombus by micro-computed tomography, at the appropriate experimental time point, dilute fibrin-targeted gold nanoparticles to a 10 milligrams per milliliter of 10 millimolar PBS concentration and sonicate the thrombus imaging agent for 30 minutes to ensure a homogeneous suspension of the nanoparticles.

Then, inject 300 microliters of the fibrin-targeted particles into the penile vein of the experimental animal. Transfer the animal to the bed of the micro-CT imager, and use a string attached to a pin to gently pull on the upper front teeth to straighten the neck. Five minutes after the gold nanoparticles have been injected, begin the imaging.