Cd63 Surface Expression
Cd63 Surface Expression - Related Videos
10:40
Characterization of Adipocyte-Derived Extracellular Vesicle Secretion Using a CD63-GFP Reporter Mouse Model In Vivo and In Vitro
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2025
The protocol uses an adipocyte-specific CD63-GFP reporter mouse line to visualize and quantify the secretion of adipocyte-derived extracellular vesicles (EVs) and demonstrate their uptake by progenitor cells, revealing a paracrine pathway within adipose tissue.
04:55
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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Cited by 6 •
2011
Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
04:39
Wireless Surface Electromyography Recording of Facial Muscle Activity During Expressions
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2025
Source:Hila Man1, Paul F. Funk2, Ieva Vebraite-Adereth3, Christoph Anders4, Orlando Guntinas-Lichius2, Yael Hanein1,3,51Sagol School of Neuroscience, Tel Aviv University, 2Department of Otorhinolaryngology, Jena University Hospital, Friedrich-Schiller-University Jena, 3School of Electrical Engineering, Tel Aviv University, 4Division Motor Research, Pathophysiology and Biomechanics, Department of Trauma, Hand and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University...
11:32
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
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Cited by 11 •
2016
Inherited cardiac arrhythmias are often caused by mutations that alter the surface delivery of one or more ion channels. Here, we adapt flow cytometry assays to provide a quantification of the relative total and cell surface protein expression of recombinant ion channels expressed in tsA-201 cells.
10:42
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
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Cited by 6 •
2017
Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.
FAQs
CD63 surface expression increases when CD63-containing late endosomal or lysosomal compartments move toward the plasma membrane and fuse with it. Fusion places CD63 on the externally accessible membrane, so the measured surface signal reflects a trafficking and membrane-remodeling event. This makes the readout useful for following vesicle mobilization rather than treating CD63 as a static intracellular marker.
Activation can increase the amount of CD63 exposed at the plasma membrane because it is associated with movement and fusion of CD63-containing compartments. Consequently, a change in surface signal can provide evidence that cellular activation is accompanied by altered vesicle mobilization or secretion. In pharmacology, this links a membrane-localization measurement to a functional response elicited by a compound.
Cd63 Surface Expression can serve as a pharmacodynamic readout because drug treatment may change vesicle mobilization, secretion, or cellular activation. Measuring the surface-associated signal after treatment allows investigators to evaluate whether a compound alters these processes at the cell membrane. The result connects drug exposure with a measurable cellular response rather than only documenting the presence of the compound.
Flow cytometry, antibody-based assays, and imaging are identified approaches for measuring CD63 at the cell surface. These methods can detect changes in the amount of surface-exposed protein, while imaging can also show where the signal appears in relation to cellular structures. Selecting among them depends on whether the study prioritizes measurement, antibody-based detection, or visual localization.
An increase in surface CD63 indicates that more CD63 has become exposed at the plasma membrane following cellular activation, vesicle mobilization, or compartment fusion. In a drug study, this change can be evaluated as evidence of altered secretion or membrane trafficking. The measurement therefore helps characterize how treatment affects cellular activity and vesicle-related responses.
Surface CD63 measurement supports extracellular vesicle research by tracking a protein associated with late endosomal and lysosomal membrane trafficking. Because these compartments can move to and fuse with the plasma membrane, their surface signal provides a measurable connection to vesicle-related cellular behavior. Pharmacology studies can use the same readout to examine drug-induced changes relevant to extracellular vesicle biology.