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Generating Chimeric Zebrafish Embryos by Transplantation
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JoVE Revista Biología
Generating Chimeric Zebrafish Embryos by Transplantation

Generating Chimeric Zebrafish Embryos by Transplantation

DOI:
10.3791/1394-v

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21:01 min

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July 17, 2009

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, ,
1HHMI and Division of Basic Sciences,Fred Hutchinson Cancer Research Center – FHCRC

Capítulos

  • 00:05Título
  • 00:13Introduction
  • 01:12Injecting Zebrafish Embryos at the 1-cell Stage
  • 06:15Preparing the Transplantation Rig and Needle
  • 08:31Transplanting Cells between Bastula Stage Embryos
  • 13:00Transplanting Primordial Germ Cells at the Blastula Stage
  • 15:02Transplating Cells Between Gastrula Stage Embryos
  • 18:46Representative Results/ Outcome
  • 20:03Conclusion

Summary

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A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.

Tags

Chimeric EmbryosZebrafishTransplantationChimera AnalysisMosaic ChimerasDonor EmbryoHost EmbryoLineage MarkerFluorescent DyeTransplantation SiteFate Maps

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